| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,134,272 | A→G | T147A (ACC→GCC) | flgK → | flagellar hook‑associated protein FlgK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,134,272 | 0 | A | G | 91.7% | 103.8 / 2.0 | 36 | T147A (ACC→GCC) | flgK | flagellar hook‑associated protein FlgK |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (14/19); total (15/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01 | |||||||||||
TACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGT > NZ_CP009273/1134139‑1134399 | tACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGAAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGAt < 2:618284/139‑1 (MQ=255)tACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGCCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGAt < 2:793676/139‑1 (MQ=255) tctTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCaa > 2:590193/1‑138 (MQ=255) cGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCt > 1:1276460/1‑139 (MQ=255) aaCACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGGGAGTAACGCGGAAGACCGGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGAc < 1:1261034/139‑1 (MQ=255) acacAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGAc > 1:590064/1‑138 (MQ=255) cAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACaaa < 1:1131183/138‑1 (MQ=255) ggATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAg > 1:710718/1‑139 (MQ=255) tcttcACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCaa > 1:146662/1‑139 (MQ=255) aCCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATcg < 2:343058/139‑1 (MQ=255) aGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACaaa < 2:177687/123‑1 (MQ=255) aGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACaaa > 1:177687/1‑123 (MQ=255) gCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGAt < 1:500521/139‑1 (MQ=255) ccTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGa > 2:500521/1‑137 (MQ=255) aaaCGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGc < 2:146662/139‑1 (MQ=255) cTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGt > 1:884633/1‑116 (MQ=255) cTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGt < 2:884633/116‑1 (MQ=255) ggTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGAc < 1:1087175/100‑1 (MQ=255) ggTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGAc > 2:1087175/1‑100 (MQ=255) aGTAACGCGGAAGCCCCGGCACCGCGCCAGGCGCTAATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTgat < 1:369848/138‑1 (MQ=255) aGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTgatc < 2:349813/139‑1 (MQ=255) aGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGAAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTgatc < 2:737584/139‑1 (MQ=255) cgcgGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTgatcagat > 2:830958/1‑138 (MQ=255) cgGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATcaa > 2:298752/1‑139 (MQ=255) aaGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATcaacaa < 2:991878/139‑1 (MQ=255) aaGACCCGGCAGCGCGCCAGGCGCTGATGGGGAAATCAGAAGGTTTGGTGAATCAGTTAAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATcaacaa < 2:729004/139‑1 (MQ=255) gACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGAt < 1:513702/69‑1 (MQ=255) gACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGAt > 2:513702/1‑69 (MQ=255) cgcCAGGCGCTGATTGGTAAATCAGAAGGATTGGTGAACCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACaaa < 1:336603/81‑1 (MQ=255) cgcCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACaaa > 2:336603/1‑81 (MQ=255) gATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCt > 1:1030284/1‑138 (MQ=255) gAAGGATTGGTGAATCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAAtct < 2:710718/139‑1 (MQ=255) gTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGa < 1:788524/139‑1 (MQ=255) tCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATa > 1:428825/1‑61 (MQ=255) tCAGTTTAAAACCGCCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATa < 2:428825/61‑1 (MQ=255) aGTTAAAAACCGCCGATCAATAGCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGGGCCAGCGTTGAGCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGAGt < 2:275780/139‑1 (MQ=255) | TACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGT > NZ_CP009273/1134139‑1134399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |