| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,199,864 | G→A | pseudogene (239/246 nt) pseudogene (8/1137 nt) |
BW25113_RS26105 → BW25113_RS05965 → |
helix‑turn‑helix domain‑containing protein terminase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,199,864 | 0 | G | A | 94.0% | 170.7 / 1.0 | 51 | pseudogene (239/246 nt) pseudogene (8/1137 nt) | BW25113_RS26105 BW25113_RS05965 | helix‑turn‑helix domain‑containing protein terminase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (21/26); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
ACGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGGTTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCACCGCCGGGTACAACAT > NZ_CP009273/1199729‑1199999 | aCGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGGtt > 1:179289/1‑138 (MQ=255)aCGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGAtt < 2:524064/138‑1 (MQ=255)aCGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGAtt < 1:1251628/138‑1 (MQ=255)aCGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGAtt < 1:923329/138‑1 (MQ=255) gAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATtcat < 2:471055/139‑1 (MQ=255) tgtgCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGGTTCATCACCCCa < 2:674834/139‑1 (MQ=255) tgtgCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCa < 2:1049808/139‑1 (MQ=255) tgtgCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCa < 1:439041/139‑1 (MQ=255) tgCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACt < 2:861422/139‑1 (MQ=255) gCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGcc > 1:244348/1‑139 (MQ=255) aTCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCATCCCACTGTGCCgtg < 2:52803/139‑1 (MQ=255) cAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCgtgg‑t > 1:1039697/1‑139 (MQ=255) cAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCgtgg‑t > 2:150756/1‑139 (MQ=255) tGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCAc > 2:248918/1‑139 (MQ=255) gTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCa < 1:150756/139‑1 (MQ=255) tCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCAc > 2:64734/1‑139 (MQ=255) gcgcGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAg < 2:1221288/138‑1 (MQ=255) cgcgGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGAt < 1:1243208/139‑1 (MQ=255) tGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTa < 2:67224/139‑1 (MQ=255) ccGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTAcacca < 1:1265037/138‑1 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCGGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCgtg < 2:848661/92‑1 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGAtt > 2:211902/1‑72 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGAtt < 1:211902/72‑1 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATtcat < 1:186972/75‑1 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATtcat > 2:186972/1‑75 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCgtg > 1:848661/1‑92 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTAcacca < 1:716795/135‑1 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTAcacca > 2:716795/1‑135 (MQ=255) tCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGAt > 1:404383/1‑139 (MQ=255) aaCGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCt > 1:331252/1‑138 (MQ=255) gCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGgcg < 1:248918/139‑1 (MQ=255) aaGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACg < 2:331252/139‑1 (MQ=255) aGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGAt < 2:1257924/94‑1 (MQ=255) aGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGAt > 1:1257924/1‑94 (MQ=255) accgcgAAGGGCGGGCCGCCGGGGTGTTATTTCTTCCCCCCAC‑GGGCCCTGGTTGGAGAAATATCCCGAGCACGCCACAAATGCGTTTTCCCCCCGGATGCTTACCGGGA‑GGGGGCGCGACGCCAGCCACTGATGTgg < 1:64734/136‑1 (MQ=255) cgcgTAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGc > 1:996771/1‑139 (MQ=255) tAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACg < 1:906319/117‑1 (MQ=255) tAAGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACg > 2:906319/1‑117 (MQ=255) aaGGCGCGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTa > 1:822784/1‑139 (MQ=255) gcgcGTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTacca < 2:1039697/139‑1 (MQ=255) cgTCGCCAGGGTGATGATTCATCACCCCACTGTGc < 1:1045637/35‑1 (MQ=255) cgTCGCCAGGGTGATGATTCATCACCCCACTGTGc > 2:1045637/1‑35 (MQ=255) cgTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCAccg < 2:822784/139‑1 (MQ=255) gTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTAc > 1:952055/1‑91 (MQ=255) gTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTAc < 2:952055/91‑1 (MQ=255) gTCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCAccgc > 2:1021486/1‑139 (MQ=255) tCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCAccgcc > 2:35603/1‑139 (MQ=255) tCGCCAGGGTGATGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCAccgcc < 2:996771/139‑1 (MQ=255) aTGGTTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCACCGCCGGGTACAACAt > 1:1240810/1‑139 (MQ=255) aTGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCACCGCCGGGTACAACAt > 2:496292/1‑139 (MQ=255) aTGATTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCACCGCCGGGTACAACAt > 2:931763/1‑139 (MQ=255) | ACGAAGGTGTGTGCTGGCCATCAATTGAAACCATTGCCCGTCAGATTGGCGCGGGGATGAGTACCGTCAGAACGGCTATCGCACGGCTGGAAGCAGAAGGCTGGTTAACGCGTAAGGCGCGTCGCCAGGGTGATGGTTCATCACCCCACTGTGCCGTGG‑TGGATGAATATCACGAGCACGCCACAGATGCGCTTTACACCACGATGCTTACCGGGATGGGGGCGCGACGCCAGCCACTGATGTGGGCCATTACCACCGCCGGGTACAACAT > NZ_CP009273/1199729‑1199999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |