| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,236,170 | A→C | L453R (CTT→CGT) | cvrA ← | potassium/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,236,170 | 0 | A | C | 96.8% | 220.6 / ‑0.7 | 62 | L453R (CTT→CGT) | cvrA | potassium/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (29/31); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01 | |||||||||||
ACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGA > NZ_CP009273/1236037‑1236304 | aCCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAAc > 2:1232619/1‑139 (MQ=255) aagaagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt < 1:956405/139‑1 (MQ=255) aagaagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt < 2:423237/139‑1 (MQ=255) aagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt < 1:313139/136‑1 (MQ=255) aagCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt > 2:313139/1‑136 (MQ=255) cGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt < 2:431545/133‑1 (MQ=255) cGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGt > 1:431545/1‑133 (MQ=255) cGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCAc > 2:569603/1‑139 (MQ=255) aTCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGaaa > 1:98975/1‑138 (MQ=255) aTCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGaaa < 1:426193/138‑1 (MQ=255) aTCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAAc < 2:988190/139‑1 (MQ=255) aTCCAGCGCGACCGGCGGCAACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGaaa < 1:326439/138‑1 (MQ=255) gcgcGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGaaa < 2:765168/133‑1 (MQ=255) gcgcGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGaaa > 1:765168/1‑133 (MQ=255) aCCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCaa > 2:592420/1‑139 (MQ=255) ggcgACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGt > 1:31116/1‑126 (MQ=255) ggcgACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGt < 2:31116/126‑1 (MQ=255) gcgACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt < 1:592420/139‑1 (MQ=255) cTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTg > 1:998210/1‑139 (MQ=255) ttACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc > 1:1069576/1‑138 (MQ=255) ccGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa < 1:1111150/138‑1 (MQ=255) gcCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa > 1:662040/1‑132 (MQ=255) gcCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa < 2:662040/132‑1 (MQ=255) ggAAGATCGCGTTCCCGACCAATTACACACAACACAGCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGGCGCAACTGGTTAGCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg < 2:874581/139‑1 (MQ=255) aGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg < 2:107367/139‑1 (MQ=255) cgTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCa < 1:269593/78‑1 (MQ=255) cgTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCa > 2:269593/1‑78 (MQ=255) ttCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACATTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACg < 1:504720/139‑1 (MQ=255) ttCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACg < 2:998210/139‑1 (MQ=255) ttCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACg < 2:1268954/139‑1 (MQ=255) ccGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa < 2:1069576/138‑1 (MQ=255) gACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCa < 2:788244/139‑1 (MQ=255) acacaacacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCa < 1:244992/139‑1 (MQ=255) acaacacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACt > 2:750868/1‑139 (MQ=255) acacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCaa < 1:569603/139‑1 (MQ=255) cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc < 1:825978/103‑1 (MQ=255) cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc > 2:825978/1‑103 (MQ=255) cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCa < 1:959082/133‑1 (MQ=255) cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCa > 2:959082/1‑133 (MQ=255) tCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa < 2:1011449/116‑1 (MQ=255) tCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa > 1:1011449/1‑116 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc < 1:186861/86‑1 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc > 2:186861/1‑86 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg > 2:739464/1‑102 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg < 1:739464/102‑1 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg > 2:892299/1‑102 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg < 1:892299/102‑1 (MQ=255) cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATaaa > 1:838326/1‑138 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg > 1:631202/1‑91 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg < 2:631202/91‑1 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATaaacaaa > 1:649795/1‑139 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATaaacaaa > 1:422135/1‑139 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt < 2:591247/67‑1 (MQ=255) cgcgCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt > 1:591247/1‑67 (MQ=255) tCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAg > 1:232652/1‑45 (MQ=255) tCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAg < 2:232652/45‑1 (MQ=255) tCGGGTGCTGCCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTcc > 2:405016/1‑139 (MQ=255) tgcCGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGAtt > 1:782369/1‑139 (MQ=255) ccGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGAtttt > 2:315657/1‑139 (MQ=255) ccGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGAtttt > 1:597745/1‑139 (MQ=255) ccGGTAGGATGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTCTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGAtttt > 1:46792/1‑139 (MQ=255) aTGACGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGa < 2:649795/139‑1 (MQ=255) | ACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGA > NZ_CP009273/1236037‑1236304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |