Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,256,207 G→A A9V (GCT→GTT)  dauA ← C4‑dicarboxylic acid transporter DauA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,256,2070GA95.6% 153.7 / ‑1.2 45A9V (GCT→GTT) dauAC4‑dicarboxylic acid transporter DauA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (24/19);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

ACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGA  >  NZ_CP009273/1256082‑1256334
                                                                                                                             |                                                                                                                               
aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCagag                                                                                                                     <  1:504282/138‑1 (MQ=255)
aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCagag                                                                                                                     >  2:504282/1‑138 (MQ=255)
aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAgc                                                                                                                    >  2:887295/1‑139 (MQ=255)
aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAgc                                                                                                                    >  2:945284/1‑139 (MQ=255)
 cTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAgcg                                                                                                                   <  1:1170327/139‑1 (MQ=255)
     ggTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGa                                                                                                               <  1:920832/139‑1 (MQ=255)
       tGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAACCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTACAGCAAACGTCGATCAGAGCGCGGa                                                                                                               >  1:594359/1‑137 (MQ=255)
         ccACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAgg                                                                                                           <  1:1090186/139‑1 (MQ=255)
         ccACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAgg                                                                                                           >  1:619743/1‑139 (MQ=255)
            caccacTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCAt                                                                                                        >  1:367062/1‑139 (MQ=255)
                 cTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACAt                                                                                                   >  1:171443/1‑139 (MQ=255)
                 cTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACAt                                                                                                   >  2:924096/1‑139 (MQ=255)
                       aTAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAg                                                                                              >  2:1086301/1‑138 (MQ=255)
                              aCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGaaa                                                                                                             >  2:94687/1‑116 (MQ=255)
                              aCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGaaa                                                                                                             <  1:94687/116‑1 (MQ=255)
                               cGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATAtt                                                                                     >  2:269144/1‑139 (MQ=255)
                                   aTCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGt                                                                                 >  1:840727/1‑139 (MQ=255)
                                    tCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGtt                                                                                >  1:935819/1‑139 (MQ=255)
                                          gCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACaat                                                                          <  1:945284/139‑1 (MQ=255)
                                                tAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGaa                                                                                                              <  2:134008/97‑1 (MQ=255)
                                                tAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGaa                                                                                                              >  1:134008/1‑97 (MQ=255)
                                                   cAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCa                                                                 <  1:269144/139‑1 (MQ=255)
                                                              aCGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGa                                                                                            <  1:297582/101‑1 (MQ=255)
                                                              aCGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGa                                                                                            >  2:297582/1‑101 (MQ=255)
                                                                        cGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCTGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCAGCCGTGAGCGCATCAtctctc                                            <  2:594359/139‑1 (MQ=255)
                                                                                  gTCATGGGTAAACCGTGCGGCAGTATATTTTTCGTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCGCATGTGATGAAAATATTTTGTTCACAATAACGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATgg                                  <  2:1205240/139‑1 (MQ=255)
                                                                                       gggTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGt                             <  2:367062/139‑1 (MQ=255)
                                                                                            aaCCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAag                         <  1:887295/138‑1 (MQ=255)
                                                                                            aaCCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAag                         >  2:480958/1‑138 (MQ=255)
                                                                                            aaCCGTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAag                         <  2:171443/138‑1 (MQ=255)
                                                                                                gTGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGc                    <  2:335571/139‑1 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGcat                                                    <  2:1044233/106‑1 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGcat                                                    >  1:1044233/1‑106 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCt                                               <  2:1061047/111‑1 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCt                                               >  1:1061047/1‑111 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCa                   >  2:1281440/1‑139 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCa                   >  2:636297/1‑139 (MQ=255)
                                                                                                 tGCGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCa                   >  2:763615/1‑139 (MQ=255)
                                                                                                   cGGCAGTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCa                   >  2:359128/1‑137 (MQ=255)
                                                                                                        gTATATTTTTCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCa            <  1:515057/139‑1 (MQ=255)
                                                                                                             tttttCTTTCCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACaa       <  1:763615/139‑1 (MQ=255)
                                                                                                                ttctttcCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATg    >  2:899001/1‑139 (MQ=255)
                                                                                                                   tttcCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGa  >  1:58907/1‑138 (MQ=255)
                                                                                                                     tcCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTc                                            >  2:1080228/1‑94 (MQ=255)
                                                                                                                     tcCAGCAAACGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTc                                            <  1:1080228/94‑1 (MQ=255)
                                                                                                                             |                                                                                                                               
ACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGA  >  NZ_CP009273/1256082‑1256334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑