| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,367,307 | T→C | V381A (GTA→GCA) | ycjN → | ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,367,307 | 0 | T | C | 94.3% | 107.6 / ‑0.4 | 35 | V381A (GTA→GCA) | ycjN | ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (16/17); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
CCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAA > NZ_CP009273/1367171‑1367430 | ccAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGcagcag < 1:1105932/139‑1 (MQ=255) tgcgctgcCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATaa > 1:654589/1‑138 (MQ=255) cgctgcCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATaaaaa > 2:755674/1‑139 (MQ=255) cgctgcCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATaaaaa > 2:167022/1‑139 (MQ=255) ccGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGcagcag > 2:556324/1‑124 (MQ=255) ccGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGcagcag < 1:556324/124‑1 (MQ=255) cGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTAc < 1:164239/139‑1 (MQ=255) aaTAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCa < 1:515776/139‑1 (MQ=255) aCTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCg < 1:1014994/122‑1 (MQ=255) aCTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCg > 2:1014994/1‑122 (MQ=255) ccACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTgg > 1:728056/1‑139 (MQ=255) gAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCgtggt < 2:1183629/138‑1 (MQ=255) gAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCgtggt < 2:654589/138‑1 (MQ=255) aaCGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGcagcag < 1:618735/82‑1 (MQ=255) aaCGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGcagcag > 2:618735/1‑82 (MQ=255) acgacgTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGTCGGGTTCTGGCGTGGTGAGTTCAAt < 1:43909/139‑1 (MQ=255) ttattaAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCa < 2:839216/139‑1 (MQ=255) cTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAg > 2:804302/1‑116 (MQ=255) cTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAg < 1:804302/116‑1 (MQ=255) cTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCg > 1:807917/1‑139 (MQ=255) aaCTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTa > 2:567749/1‑138 (MQ=255) ccGAATCAGCT‑ATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc < 2:68632/137‑1 (MQ=255) cGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCAcc > 1:709159/1‑125 (MQ=255) cGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCAcc < 2:709159/125‑1 (MQ=255) cGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGa > 2:907544/1‑139 (MQ=255) cGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGa < 2:70560/139‑1 (MQ=255) cGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGa > 1:608673/1‑139 (MQ=255) aaTCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtc > 1:249153/1‑139 (MQ=255) aTCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCCGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGTTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtct < 1:551942/139‑1 (MQ=255) aTCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAtct < 2:728056/139‑1 (MQ=255) tGAACTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTg > 2:631658/1‑139 (MQ=255) cTGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAg < 1:1077379/139‑1 (MQ=255) tGCCAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGc > 2:588241/1‑139 (MQ=255) ccAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGt < 2:610564/104‑1 (MQ=255) ccAAATATTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGt > 1:610564/1‑104 (MQ=255) tatTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCa > 1:164799/1‑87 (MQ=255) tatTCAGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCa < 2:164799/87‑1 (MQ=255) aGGTTTTTGGCGCAGCAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGaaaaa < 2:608673/139‑1 (MQ=255) tGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc > 2:1009071/1‑97 (MQ=255) tGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc < 1:1009071/97‑1 (MQ=255) | CCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAGCCAGAAAAA > NZ_CP009273/1367171‑1367430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |