Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,213,874	Δ1 bp	coding (1091/1524 nt)	pdeG →	cyclic diguanylate phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,213,874	0	G	.	100.0%	103.9 / NA	24	coding (1091/1524 nt)	pdeG	cyclic diguanylate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/11);  total (13/11)

ATGAACGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTTCGG  >  NZ_CP009273/1213753‑1213997                                                                                                                          |                                                                                                                            aTGAACGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGt                                                                                                           >  1:9138/1‑139 (MQ=255)                aGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCAcc                                                                                              >  2:838744/1‑137 (MQ=255)                aGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCAcc                                                                                              >  2:517523/1‑137 (MQ=255)                        gCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg                                                                                     <  1:702195/138‑1 (MQ=255)                          cGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCa                                                                                  <  2:133481/139‑1 (MQ=255)                            gAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCAtt                                                                                <  2:182045/139‑1 (MQ=255)                                  tttCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGt                                                                          >  1:607072/1‑139 (MQ=255)                                          tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGt                                                                          <  1:811423/131‑1 (MQ=255)                                          tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGt                                                                          >  2:811423/1‑131 (MQ=255)                                           ggAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAg                                                                 >  1:830872/1‑139 (MQ=255)                                                      tAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg                                                       <  2:106313/138‑1 (MQ=255)                                                      tAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAg                                                       <  2:804410/138‑1 (MQ=255)                                                              cGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGt                                                   >  1:269602/1‑134 (MQ=255)                                                              cGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGt                                                   <  2:269602/134‑1 (MQ=255)                                                                          cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGa                                   >  2:565403/1‑138 (MQ=255)                                                                          cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGa                                   >  2:828929/1‑138 (MQ=255)                                                                                cGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGt                            <  2:592067/139‑1 (MQ=255)                                                                                             gtgtTTAAATTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCAc               >  2:799271/1‑139 (MQ=255)                                                                                                   aaaTTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGgat          <  1:517523/138‑1 (MQ=255)                                                                                                   aaaTTTCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGgat          <  1:522084/138‑1 (MQ=255)                                                                                                        tCCGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTTc    >  2:387264/1‑139 (MQ=255)                                                                                                          cGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCAt                    >  2:534890/1‑121 (MQ=255)                                                                                                          cGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCAt                    <  1:534890/121‑1 (MQ=255)                                                                                                          cGTGACAGTTTTACCC‑CCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTTCgg  >  1:164881/1‑139 (MQ=255)                                                                                                                          |                                                                                                                            ATGAACGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATACTGCATGAAAATGGTTTTGTCATCGCACTGGATGATTTCGG  >  NZ_CP009273/1213753‑1213997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑