Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,590,869	Δ1 bp	coding (109/714 nt)	purC ←	phosphoribosylaminoimidazolesuccinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,590,868	0	C	.	100.0%	202.1 / NA	45	coding (110/714 nt)	purC	phosphoribosylaminoimidazolesuccinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/26);  total (19/26)

TCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGT  >  NZ_CP009273/2590734‑2590996                                                                                                                                       |                                                                                                                                 tCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgcgcatcc                                                                                                                              >  1:653879/1‑135 (MQ=255) tCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgcgcatc                                                                                                                               <  1:389407/138‑4 (MQ=255)             cGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTa                                                                                                                 >  2:238297/1‑139 (MQ=255)                          cAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAAtt                                                                                                    >  1:780021/1‑139 (MQ=255)                          cAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAAtt                                                                                                    >  1:410107/1‑139 (MQ=255)                             aCGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTc                                                                                                   >  1:414633/1‑137 (MQ=255)                               gCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGc                                                                                               <  1:136040/139‑1 (MQ=255)                                          gTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTc                                                                                    <  1:238297/139‑1 (MQ=255)                                           tCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTcc                                                                                   <  1:828024/139‑1 (MQ=255)                                             gggATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGa                                                                                                 <  2:287688/123‑1 (MQ=255)                                             gggATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGa                                                                                                 >  1:287688/1‑123 (MQ=255)                                             gggATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCgg                                                                                 <  1:423710/139‑1 (MQ=255)                                                      gCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGt                                                                        <  2:414633/139‑1 (MQ=255)                                                      gCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGt                                                                        <  1:795829/139‑1 (MQ=255)                                                       cTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       <  2:410107/139‑1 (MQ=255)                                                        ttCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgcgcatcc                                                                                                                              <  2:190096/84‑5 (MQ=255)                                                        ttCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATgcgcgcgcatcc                                                                                                                              >  1:190096/1‑80 (MQ=255)                                                         tCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCt                                                                     >  1:726385/1‑139 (MQ=255)                                                          cAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGc                                                                      <  1:112693/137‑1 (MQ=255)                                                                           aTGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCtt                                                    >  2:679630/1‑138 (MQ=255)                                                                              aaGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTAc                                                 <  2:653879/138‑1 (MQ=255)                                                                                  agtTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       <  1:810035/112‑1 (MQ=255)                                                                                  agtTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       >  2:810035/1‑112 (MQ=255)                                                                                   gtTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGa                                            <  1:304128/138‑1 (MQ=255)                                                                                    ttGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATa                                          >  1:828470/1‑139 (MQ=255)                                                                                       aaCTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACaa                                       <  2:780021/139‑1 (MQ=255)                                                                                            gttgttCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAg                                  >  1:179479/1‑139 (MQ=255)                                                                                                      aTACCTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTg                        >  2:128622/1‑139 (MQ=255)                                                                                                          cTTTGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATc                    <  2:726385/139‑1 (MQ=255)                                                                                                            ttGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       <  1:761735/86‑1 (MQ=255)                                                                                                            ttGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       >  2:761735/1‑86 (MQ=255)                                                                                                            ttGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCtt                  >  2:774717/1‑139 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTg                                                                       <  2:379956/85‑1 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc                       >  1:39099/1‑133 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc                       <  2:39099/133‑1 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc                       <  2:504137/133‑1 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc                       >  1:504137/1‑133 (MQ=255)                                                                                                             tGCGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGCTTTCCGTg                                                                       >  1:379956/1‑85 (MQ=255)                                                                                                               cGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTa                <  2:779950/138‑1 (MQ=255)                                                                                                               cGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTa                <  2:828470/138‑1 (MQ=255)                                                                                                               cGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTa                <  1:128622/138‑1 (MQ=255)                                                                                                               cGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt               <  2:511927/139‑1 (MQ=255)                                                                                                               cGATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt               <  2:438655/139‑1 (MQ=255)                                                                                                                gATCAAACTGCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATc              <  1:679630/139‑1 (MQ=255)                                                                                                                         gCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCa                                               <  2:21676/97‑1 (MQ=255)                                                                                                                         gCTCAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCa                                               >  1:21676/1‑97 (MQ=255)                                                                                                                            cAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGgtgt  >  2:512676/1‑139 (MQ=255)                                                                                                                            cAATGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGgtgt  >  2:297997/1‑139 (MQ=255)                                                                                                                             aaTGCGCGCG‑CATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGgtg   <  2:179479/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 TCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGATCAAACTGCTCAATGCGCGCGCCATCCCCTGCTGACGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGT  >  NZ_CP009273/2590734‑2590996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑