New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	= 3633951	58 (1.390)	5 (0.150)	5/192	2.7	9.2%	intergenic (+46/+21)	uspA/BW25113_RS26225	universal stress protein UspA/hypothetical protein
	?	NZ_CP009273	= 3633929	51 (1.500)					intergenic (+24/+43)	uspA/BW25113_RS26225	universal stress protein UspA/hypothetical protein
Rejected: Frequency below/above cutoff threshold.

TCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTCCATCATTAGTGTGATCATCTGG  >  NZ_CP009273/3633814‑3634087                                                                                                                                          |                                                                                                                                         tCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGttt                                                                                                                                         >  2:569502/1‑139 (MQ=255)               aTGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgcg                                                                                                                           <  1:1491/139‑1 (MQ=255)               aTGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc                                                                                                                            <  2:583831/138‑1 (MQ=255)               aTGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc                                                                                                                            <  2:263660/138‑1 (MQ=255)               aTGTCTGCCGCACGTCAGCTGAGCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcg                                                                                                                             <  2:194115/137‑1 (MQ=255)                         cACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTAcc                                                                                                                 >  1:695071/1‑139 (MQ=255)                         cACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTAcc                                                                                                                 >  1:689889/1‑139 (MQ=255)                         cACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTAcc                                                                                                                 >  2:793075/1‑139 (MQ=255)                            gTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGa                                                                                                              >  1:412841/1‑139 (MQ=255)                               aGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            <  1:206670/138‑1 (MQ=255)                               aGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            <  2:212427/138‑1 (MQ=255)                                gCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAg                                                                                                          <  2:695071/139‑1 (MQ=255)                                     tCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTaa                                                                                                     <  1:684692/139‑1 (MQ=255)                                         cacCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAacac                                                                                                 <  1:476436/139‑1 (MQ=255)                                         cacCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAacac                                                                                                 <  2:437412/139‑1 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTAc                                                                                                                  >  1:306825/1‑117 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTAc                                                                                                                  <  2:306825/117‑1 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            >  1:82295/1‑123 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            <  2:82295/123‑1 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTAt                                                                                            >  1:651511/1‑139 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTAt                                                                                            >  1:654468/1‑139 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTAt                                                                                            >  1:310578/1‑139 (MQ=255)                                              ttCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTAt                                                                                            <  2:689889/139‑1 (MQ=255)                                                cACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTcaggaac                                                                                                                                   >  1:177807‑M1/1‑91 (MQ=255)                                                 aCGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTCCCTGATCAGCGTAACCACCTTATCt                                                                                          <  1:329594/138‑1 (MQ=255)                                                   gTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGc                                                                                       >  1:259294/1‑139 (MQ=255)                                                             tGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa                                                                                                      >  1:629009/1‑114 (MQ=255)                                                             tGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTa                                                                                                      <  2:629009/114‑1 (MQ=255)                                                               aTTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTcaggaacacgtcgtagagggaagattattcttcttcgtcgcgcagcggaacaatcagcatat                                                                            >  1:425984‑M1/1‑76 (MQ=255)                                                               aTTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCg                                                                           >  1:354768/1‑139 (MQ=255)                                                                 tGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt                                                                                                                       <  2:314827/93‑1 (MQ=255)                                                                 tGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCtt                                                                                                                       >  1:314827/1‑93 (MQ=255)                                                                 tGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTa                                                                         >  2:294761/1‑139 (MQ=255)                                                                       gCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTcaggaacacgtcgtagagggaagattattcttcttcgtcgcgcagcggaacaatcagcatatcaacgtgaa                                                                   <  1:136543‑M1/139‑72 (MQ=255)                                                                           cgcgACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc                                                                                                                            >  1:107260/1‑78 (MQ=255)                                                                           cgcgACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc                                                                                                                            <  2:107260/78‑1 (MQ=255)                                                                                   agaagaaTAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACgg                                                                                 <  2:166025/113‑1 (MQ=255)                                                                                   agaagaaTAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACgg                                                                                 >  1:166025/1‑113 (MQ=255)                                                                                     aagaaTAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGc                                                      <  2:308001/138‑1 (MQ=255)                                                                                     aagaaTAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGc                                                      >  1:308001/1‑138 (MQ=255)                                                                                      agaaTAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTcaggaacacgtcgtagagggaagattattcttcttcgtcgcgcagcggaacaatcagcatatcaacgtgaacggtgttgatcagct                                                    >  1:352440‑M1/1‑53 (MQ=255)                                                                                         ataatCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATa                                                 <  1:793075/139‑1 (MQ=255)                                                                                            atCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATaca                                               <  2:654468/138‑1 (MQ=255)                                                                                            atCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATaca                                               <  1:432864/138‑1 (MQ=255)                                                                                             tCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcg                                                                                                                             <  1:760260/59‑1 (MQ=255)                                                                                             tCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcg                                                                                                                             >  2:760260/1‑59 (MQ=255)                                                                                               ttCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            <  1:573891/74‑1 (MQ=255)                                                                                               ttCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATc                                                                                                            >  2:573891/1‑74 (MQ=255)                                                                                                  ccTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGt                                                                                >  1:231044/1‑99 (MQ=255)                                                                                                  ccTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGt                                                                                <  2:231044/99‑1 (MQ=255)                                                                                                           cgTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACcatca                               >  1:441754/1‑139 (MQ=255)                                                                                                              gtTCCTGAACGCCCGCATATGCGGGCGTTcaggaacacgtcgtagagggaagattattcttcttcgtcgcgcagcggaacaatcagcatatcaacgtg                                                                     >  2:177807‑M1/1‑29 (MQ=255)                                                                                                                tCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATAttt                          >  2:124531/1‑139 (MQ=255)                                                                                                                  cTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTc                        <  2:441754/139‑1 (MQ=255)                                                                                                                  cTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTc                        <  2:759053/139‑1 (MQ=255)                                                                                                                  cTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTc                        <  2:698546/139‑1 (MQ=255)                                                                                                                       cGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgcgc                                                                                                                          >  1:428277/1‑36 (MQ=255)                                                                                                                       cGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgcgc                                                                                                                          <  2:428277/36‑1 (MQ=255)                                                                                                                           cGCATATGCGGGCGTTcaggaacacgtcgtagagggaagattattcttcttcgtcgcgcagcggaacaatcagcatatcaacgtgaacggtgttgatcagctgacgtgcggaagacatcagtttgctccagaagtcctg               <  2:90431‑M1/139‑124 (MQ=255)                                                                                                                                    gggCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATaca                                               <  2:687798/98‑1 (MQ=255)                                                                                                                                    gggCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATaca                                               >  1:687798/1‑98 (MQ=255)                                                                                                                                    gggCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTaa                                       >  1:198699/1‑106 (MQ=255)                                                                                                                                    gggCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTaa                                       <  2:198699/106‑1 (MQ=255)                                                                                                                                        gTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTCCATCATTAGTGTGATCATCTgg  >  2:805989/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                         TCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCAGCGTAAACACCTTATCTGGCCTACGGTCTGCGTACGCAATCAAAATCCCCAGCCAATACAACATTTAACACCATCATATTTTCCATCATTAGTGTGATCATCTGG  >  NZ_CP009273/3633814‑3634087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.