| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 146,045 | 0 | T | C | 62.5% | 34.3 / 54.6 | 48 | E15G (GAA→GGA) | panB | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/7); new base C (16/14); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCA > NZ_CP009273/145916‑146177 | aGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTg > 1:523553/1‑138 (MQ=255) aGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTg < 2:701890/139‑1 (MQ=255) aGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTg < 2:41807/139‑1 (MQ=255) tGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGt > 1:408038/1‑138 (MQ=255) tGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGt > 2:428288/1‑138 (MQ=255) tCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt < 2:347894/139‑1 (MQ=255) tCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt > 1:347894/1‑139 (MQ=255) tCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt > 1:475620/1‑139 (MQ=255) aTGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTg > 2:789020/1‑111 (MQ=255) aTGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTg < 1:789020/111‑1 (MQ=255) tGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCgg < 2:225275/139‑1 (MQ=255) cccAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGtt > 2:193666/1‑139 (MQ=255) cGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCAt > 1:752261/1‑138 (MQ=255) cccACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATc < 1:193666/139‑1 (MQ=255) aaGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGAtgttg > 1:223076/1‑139 (MQ=255) gCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 2:408038/139‑1 (MQ=255) gCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 2:752261/139‑1 (MQ=255) ttCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt > 1:78256/1‑99 (MQ=255) ttCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATggtggt < 2:78256/99‑1 (MQ=255) tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCa > 2:554305/1‑82 (MQ=255) tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCa < 1:554305/82‑1 (MQ=255) tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTc > 2:243607/1‑139 (MQ=255) cAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACtt < 2:847982/70‑1 (MQ=255) cAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACtt > 1:847982/1‑70 (MQ=255) gagTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt < 1:428288/139‑1 (MQ=255) gagTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt < 1:243607/139‑1 (MQ=255) agTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt > 2:461667/1‑138 (MQ=255) ggCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCa > 1:835739/1‑127 (MQ=255) ggCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCa < 2:835739/127‑1 (MQ=255) cGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTcaca < 1:814667/139‑1 (MQ=255) aTAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCaa > 1:301613/1‑139 (MQ=255) aTAAGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCa < 2:585235/138‑1 (MQ=255) aaGCGGTGATGGTCGCGAAACGTTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt > 1:700424/1‑139 (MQ=255) aaGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt > 2:547114/1‑138 (MQ=255) aaGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt > 1:305060/1‑138 (MQ=255) aaGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt > 2:658906/1‑138 (MQ=255) aaGCGGTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt > 1:569751/1‑138 (MQ=255) aGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 2:755346/99‑1 (MQ=255) aGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg > 1:755346/1‑99 (MQ=255) gTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTa > 1:638801/1‑139 (MQ=255) gTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc > 1:676490/1‑66 (MQ=255) gTGATGGTCGCGAAACG‑TTTTTTTCCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc < 2:676490/66‑1 (MQ=255) tGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTAt > 1:88303/1‑139 (MQ=255) aTGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATc < 2:447528/138‑1 (MQ=255) cgcgAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCt > 1:119190/1‑93 (MQ=255) cgcgAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCt < 2:119190/93‑1 (MQ=255) gAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagcagc < 2:638801/139‑1 (MQ=255) gAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagcag > 2:544373/1‑138 (MQ=255) tttttcCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCa < 2:281834/138‑1 (MQ=255) | AGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACG‑TTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCA > NZ_CP009273/145916‑146177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |