Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273185,0640AC67.4% 47.0 / 42.3 43V25G (GTC→GGC) glnDbifunctional uridylyltransferase/uridylyl‑removing protein GlnD
Reads supporting (aligned to +/- strand):  ref base A (6/8);  new base C (11/18);  total (17/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCGGCTT  >  NZ_CP009273/184931‑185194
                                                                                                                                     |                                                                                                                                  
gTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCAt                                                                                                                               >  2:157173/1‑139 (MQ=255)
             gTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGtt                                                                                                                  >  1:257922/1‑139 (MQ=255)
                cgcgcCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGTTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGa                                                                                                                <  2:281358/138‑1 (MQ=255)
                cgcgcCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGa                                                                                                                <  2:139209/138‑1 (MQ=255)
                cgcgcCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGAc                                                                                                               <  2:664972/139‑1 (MQ=255)
                   gcCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCg                                                                                                                     >  1:198021/1‑130 (MQ=255)
                   gcCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCg                                                                                                                     <  2:198021/130‑1 (MQ=255)
                    ccTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCggg                                                                                                           <  2:734300/139‑1 (MQ=255)
                     cTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGc                                                                                                          <  1:445085/139‑1 (MQ=255)
                     cTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGc                                                                                                          <  1:684348/139‑1 (MQ=255)
                     cTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGc                                                                                                          <  2:257922/139‑1 (MQ=255)
                                 ttCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTggg                                                                                                  >  2:826192/1‑135 (MQ=255)
                                 ttCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTggg                                                                                                  <  1:826192/135‑1 (MQ=255)
                                   cTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGagag                                                                                             <  2:675767/138‑1 (MQ=255)
                                    tGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCg                                                                                           >  2:281920/1‑139 (MQ=255)
                                      cAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCgg                                                                                          <  1:190065/138‑1 (MQ=255)
                                           aTCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCggg                                                                                                           <  2:309820/116‑1 (MQ=255)
                                           aTCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCggg                                                                                                           >  1:309820/1‑116 (MQ=255)
                                           aTCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGc                                                                                                          >  1:473575/1‑117 (MQ=255)
                                           aTCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGc                                                                                                          <  2:473575/117‑1 (MQ=255)
                                              ccATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCgg                                                                                          >  1:502592/1‑130 (MQ=255)
                                              ccATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCgg                                                                                          <  2:502592/130‑1 (MQ=255)
                                               cATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTa                                                                                <  1:157173/139‑1 (MQ=255)
                                               cATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTa                                                                                <  2:709621/139‑1 (MQ=255)
                                                   gTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTg                                                                             <  1:211929/138‑1 (MQ=255)
                                                         ggCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGa                                                                      <  1:784650/139‑1 (MQ=255)
                                                              cACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAggg                                                                  >  1:389026/1‑138 (MQ=255)
                                                                          gCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTg                                                                        >  1:760426/1‑120 (MQ=255)
                                                                          gCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTg                                                                        <  2:760426/120‑1 (MQ=255)
                                                                          gCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTgcg                                                     <  1:281920/139‑1 (MQ=255)
                                                                                     tCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCa                                              <  1:779942/135‑1 (MQ=255)
                                                                                     tCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCa                                              >  2:779942/1‑135 (MQ=255)
                                                                                             ggCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAAt                                   >  1:416822/1‑138 (MQ=255)
                                                                                                       ccACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATtgtg                                                       <  2:706997/108‑1 (MQ=255)
                                                                                                       ccACCGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATtgtg                                                       >  1:706997/1‑108 (MQ=255)
                                                                                                          ccGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTa                                                                                <  2:745801/80‑1 (MQ=255)
                                                                                                          ccGACGGTTAATTCATCACGGGGCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTa                                                                                >  1:745801/1‑80 (MQ=255)
                                                                                                                          caCGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCgg     >  2:423797/1‑139 (MQ=255)
                                                                                                                             ggggCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTgcg                                                     >  1:846592/1‑88 (MQ=255)
                                                                                                                             ggggCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTgcg                                                     <  2:846592/88‑1 (MQ=255)
                                                                                                                             ggggCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCGGCtt  <  2:539380/139‑1 (MQ=255)
                                                                                                                               ggCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCGGCt   <  2:57198/136‑1 (MQ=255)
                                                                                                                               ggCCAGCCGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCGGCt   >  1:57198/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                  
GTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACGCTGGAAAGTATCGATATGGGCTTTTATCCCACCGACGGTTAATTCATCACGGGGCCAGACGCATGGATTTTGCGGTTGACCGGGCAGGGTGGGGAGAGCGGTGTTTGCGTACTGTTCTGGAAGGGTATTCATTGTGCGCCACCCATAAGATTAAATTATCGCATTAAAAAAGCCGGCATTCGCCGGCTT  >  NZ_CP009273/184931‑185194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑