Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273186,7070AT67.7% 42.0 / 29.1 31D116V (GAC→GTC) rpsB30S ribosomal protein S2
Reads supporting (aligned to +/- strand):  ref base A (3/7);  new base T (10/11);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCGCTGCAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCGGTATCAAAGACATGGGCGGTCTGCCGGACG  >  NZ_CP009273/186579‑186838
                                                                                                                                |                                                                                                                                   
aCGCGCTGCAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACt                                                                                                                           <  1:87834/139‑1 (MQ=255)
 cgcgCTGCAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTc                                                                                                                          <  2:440187/139‑1 (MQ=255)
        cAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGtct                                                                                                                     <  1:165993/137‑1 (MQ=255)
        cAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGtct                                                                                                                     <  2:253865/137‑1 (MQ=255)
                gcgGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTa                                                                                                           <  1:811546/139‑1 (MQ=255)
                           cGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAg                                                                                                <  1:76611/139‑1 (MQ=255)
                                ctctGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGAc                                                                                           >  2:606570/1‑139 (MQ=255)
                                          cGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCaagaaag                                                                                   >  2:758318/1‑137 (MQ=255)
                                          cGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCaagaaag                                                                                   >  2:41650/1‑137 (MQ=255)
                                          cGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCaagaaag                                                                                   >  1:215962/1‑137 (MQ=255)
                                                      cGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGtct                                                                                                                     >  1:234858/1‑91 (MQ=255)
                                                      cGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGtct                                                                                                                     <  2:234858/91‑1 (MQ=255)
                                                        tGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCAc                                                                   <  2:774176/139‑1 (MQ=255)
                                                               tCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATgcg                                                                      >  2:21279/1‑129 (MQ=255)
                                                               tCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATgcg                                                                      <  1:21279/129‑1 (MQ=255)
                                                                     gctggGCGGTATGCTGACTAACTGGAAAACACTCCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCCAGAAAGAAGCGCTGATGCGCACTCGTGAGCTg                                                         >  2:203066/1‑136 (MQ=255)
                                                                               aTGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGAc                                                                                           >  2:187349/1‑92 (MQ=255)
                                                                               aTGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGAc                                                                                           <  1:187349/92‑1 (MQ=255)
                                                                                        aaCTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATgcg                                                                      <  1:665397/104‑1 (MQ=255)
                                                                                        aaCTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATgcg                                                                      >  2:665397/1‑104 (MQ=255)
                                                                                         aCTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTggg                                  <  1:41650/139‑1 (MQ=255)
                                                                                          cTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGc                                 >  2:185314/1‑139 (MQ=255)
                                                                                          cTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGc                                 >  1:44399/1‑139 (MQ=255)
                                                                                             gAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                <  1:173176/137‑1 (MQ=255)
                                                                                             gAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                <  2:44399/137‑1 (MQ=255)
                                                                                             gAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                <  1:844195/137‑1 (MQ=255)
                                                                                             gAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                <  1:757414/137‑1 (MQ=255)
                                                                                               aaaCCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                >  2:662816/1‑135 (MQ=255)
                                                                                               aaaCCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCg                                <  1:662816/135‑1 (MQ=255)
                                                                                                     ttCGTCAGTCCATCAAACGTCTGAAAGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCGGTATCAAAGa                      >  2:351083/1‑139 (MQ=255)
                                                                                                                            aaaGTCCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCGGTATCAAAGACATGGGCGGTCTGCCGGACg  <  1:185314/136‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
ACGCGCTGCAAGCGAAGCGGTGAAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATGCTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAGTCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAGCTGGAGAAACTGGAAAACAGCCTGGGCGGTATCAAAGACATGGGCGGTCTGCCGGACG  >  NZ_CP009273/186579‑186838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑