| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,591 | 0 | T | C | 66.7% | ‑4.5 / 15.3 | 18 | K271E (AAA→GAA) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base T (6/0); new base C (0/12); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAC > NZ_CP009273/353453‑353723 | gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAttttt > 1:302014/1‑139 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg > 1:30489/1‑139 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgccg > 1:25749/1‑139 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac > 2:177429/1‑138 (MQ=255) ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc > 2:120635/1‑139 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtag > 2:601335/1‑139 (MQ=255) ccTGTGTCAGGGCCTCCCGTGATTCTTTCCCCCCAACACCCCCGCATTTATCCAACCCCAT‑GTGAAAAAATGCCTTCGGGGCGGCTGTCGGCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTgg < 1:620572/139‑1 (MQ=255) cTGTGTCAGCGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCAACGCCTG‑GTGAAAAAATGCCTTCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc < 1:601335/139‑1 (MQ=18) ccggcgcCTACCGTGATTCATTCCCCCCAACACCCGCGTATTTA‑CCAACCCCCTGGTAAAAAAATCCCTCCGCGGCGGCTGTCTGCCAGCTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 2:35319/136‑1 (MQ=16) cAGCGCCTCCCGTGATTCATTCCCCCCAACACCCGCGCTTTTACCCAACGCCAT‑GTAAAAAAATGCTGCCGCGGCGGCTTTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 2:670294/139‑1 (MQ=255) attccccGCCACCACCCGCGCTTTTCCCCACCGCCAT‑GTCAAAAAATGCCTCCGCGGGGGCTTTCTGCCATCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTaa < 2:51128/133‑1 (MQ=255) tttcccGCCAACACCCCCGCATTTACCCACCGCCAT‑GTGAAAAAGTGCCTCCGGGGGGGTTGTCGGCCATCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc < 2:712302/135‑1 (MQ=255) aTTCCCGCAAACACCCGCGCATTTACCCAACGCCAT‑GTGAAAAATGGCCTTCGCGGGGGCTGTCTGCCAGCTATTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGc < 1:8531/139‑1 (MQ=255) cgcATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAGAAAAATTTTAACCCGTCCTCTTGTGTggg > 1:715208/1‑138 (MQ=37) aTTTACCCACCGCCAT‑GTCCAAAAAGCCCTTCGGGGCGGCTGTCTGCCAGCTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt < 1:678915/139‑1 (MQ=255) tacccAACCCCTT‑GTGCAAAAATCCCTCCGGGGCGGCTGTCTGCCGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCg < 2:302014/135‑1 (MQ=255) gTCTCCCAGTTTTTTCCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc < 2:760580/138‑1 (MQ=255) tCTGCCAGTTTTTTTCCCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc < 1:672022/137‑1 (MQ=255) ccTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc < 2:243055/136‑1 (MQ=255) tttttCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc < 1:736332/137‑1 (MQ=255) | GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCAT‑GTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAC > NZ_CP009273/353453‑353723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |