Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,662,8140GA65.8% 47.3 / 39.8 41A3V (GCT→GTT) mlcDNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (3/11);  new base A (14/13);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 9.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACATTTTTCGGGCTTTTTCGCT  >  NZ_CP009273/1662679‑1662945
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gTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCaa                                                                                                                                  <  2:788303/139‑1 (MQ=255)
     aCTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAaccacc                                                                                                                             >  1:147557/1‑139 (MQ=255)
         gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCa                                                                                                                          <  1:517022/138‑1 (MQ=255)
         gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCa                                                                                                                          <  2:147557/138‑1 (MQ=255)
         gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAacc                                                                                                                                >  2:417483/1‑132 (MQ=255)
         gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAacc                                                                                                                                <  1:417483/132‑1 (MQ=255)
          cAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATa                                                                                                                        <  2:173374/139‑1 (MQ=255)
          cAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATa                                                                                                                        <  1:465875/139‑1 (MQ=255)
          cAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATa                                                                                                                        <  1:602366/139‑1 (MQ=255)
               gCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATa                                                                                                                        <  2:706516/134‑1 (MQ=255)
               gCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATa                                                                                                                        >  1:706516/1‑134 (MQ=255)
                   gTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCtata                                                                                                               >  1:757337/1‑139 (MQ=255)
                                      aTCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTcaacaa                                                                                                                                  >  1:2399/1‑101 (MQ=255)
                                      aTCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTcaacaa                                                                                                                                  <  2:2399/101‑1 (MQ=255)
                                         gATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCt                                                                                         >  1:266147/1‑139 (MQ=255)
                                             cgcgAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTa                                                                                      <  2:757337/138‑1 (MQ=255)
                                                 agaCTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTa                                                                                 <  1:67450/139‑1 (MQ=255)
                                                     tGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGt                                                                             >  2:10112/1‑139 (MQ=255)
                                                       gACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTg                                                                            <  1:259671/138‑1 (MQ=255)
                                                         ccAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAg                                                                         <  2:231990/139‑1 (MQ=255)
                                                         ccAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAg                                                                         <  1:57095/139‑1 (MQ=255)
                                                         ccAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAg                                                                         <  1:506441/139‑1 (MQ=255)
                                                         ccAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAg                                                                         <  2:266147/139‑1 (MQ=255)
                                                            aGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCAc                                                                      >  2:145986/1‑139 (MQ=255)
                                                            aGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCAc                                                                      >  1:8048/1‑139 (MQ=255)
                                                            aGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCAc                                                                      >  1:246613/1‑139 (MQ=255)
                                                            aGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCAc                                                                      <  1:645963/139‑1 (MQ=255)
                                                                 atcaatcaGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAg                                                                                                                                     >  2:660813/1‑71 (MQ=255)
                                                                 atcaatcaGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAg                                                                                                                                     <  1:660813/71‑1 (MQ=255)
                                                                           cGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTgtcgt                                                       >  1:828239/1‑139 (MQ=255)
                                                                            gATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTgtcgtc                                                      <  1:10112/139‑1 (MQ=255)
                                                                                    gcgcCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTcc                                                                                                                    >  1:39366/1‑69 (MQ=255)
                                                                                    gcgcCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTcc                                                                                                                    <  2:39366/69‑1 (MQ=255)
                                                                                    gcgcCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAAtttt                                               >  2:104247/1‑138 (MQ=255)
                                                                                       cccGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACt                                           <  1:181334/139‑1 (MQ=255)
                                                                                         cgcgTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTa                                         <  1:555693/139‑1 (MQ=255)
                                                                                            gTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGc                                      <  1:801261/139‑1 (MQ=255)
                                                                                            gTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGc                                      <  2:666458/139‑1 (MQ=255)
                                                                                                        tATTTGATCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTaa                            <  2:246613/137‑1 (MQ=255)
                                                                                                               tCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACt                                           <  2:82861/115‑1 (MQ=255)
                                                                                                               tCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACt                                           >  1:82861/1‑115 (MQ=255)
                                                                                                               tCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACAtt                   >  2:851427/1‑139 (MQ=255)
                                                                                                               tCAATGTGCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACAtt                   >  2:359094/1‑139 (MQ=255)
                                                                                                                    gtgCCCAGGCTGGTTTTCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACATTTTTCg              >  1:306216/1‑139 (MQ=255)
                                                                                                                                 ttttCAACAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACATTTTTCGGGCTTTTTCGCt  >  2:142316/1‑138 (MQ=255)
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GTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGGTGAAGCACTTCAATACGTGTCGTCAAATTTTTACTTAGGCATGTGATTAACAGCACATTTTTCGGGCTTTTTCGCT  >  NZ_CP009273/1662679‑1662945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑