Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,525,5460TC66.7% 54.5 / 53.0 54V242A (GTC→GCC) cysZsulfate transporter CysZ
Reads supporting (aligned to +/- strand):  ref base T (7/11);  new base C (16/20);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-04
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTAT  >  NZ_CP009273/2525413‑2525679
                                                                                                                                     |                                                                                                                                     
aTGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGAtt                                                                                                                                  >  1:772811/1‑139 (MQ=255)
 tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTg                                                                                                                                 >  1:545152/1‑139 (MQ=255)
 tGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTg                                                                                                                                 >  2:588363/1‑139 (MQ=255)
     cACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTAt                                                                                                                             <  2:545152/139‑1 (MQ=255)
        cGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATcgc                                                                                                                          >  2:169051/1‑139 (MQ=255)
          cccTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGa                                                                                                                        >  2:781419/1‑139 (MQ=255)
          cccTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGa                                                                                                                        >  2:114416/1‑139 (MQ=255)
          cccTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGa                                                                                                                        >  2:348100/1‑139 (MQ=255)
           ccTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGAt                                                                                                                       <  1:829997/139‑1 (MQ=255)
             tGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATa                                                                                                                      >  2:59228/1‑138 (MQ=255)
               cgcACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATaaa                                                                                                                    >  1:554708/1‑138 (MQ=255)
               cgcACACGCAAAATCACCAATATGCAGACTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATaaa                                                                                                                    >  1:382705/1‑138 (MQ=255)
                gcACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAaca                                                                                                                  <  2:337424/139‑1 (MQ=255)
                    acGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACAcgc                                                                                                               >  2:413349/1‑138 (MQ=255)
                         aaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGAtgt                                                                                                          <  1:266005/138‑1 (MQ=255)
                         aaaTCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGAtgt                                                                                                          >  2:266005/1‑138 (MQ=255)
                              aCCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGTTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGt                                                                                                    <  2:647085/139‑1 (MQ=255)
                                       cAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCt                                                                                            <  1:413349/138‑1 (MQ=255)
                                          tttGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAAAAATCTACCg                                                                                        >  2:37576/1‑139 (MQ=255)
                                                    tAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTg                                                                               >  2:272785/1‑138 (MQ=255)
                                                           ccTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGAtgtg                                                                                                         >  2:216506/1‑105 (MQ=255)
                                                           ccTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGAtgtg                                                                                                         <  1:216506/105‑1 (MQ=255)
                                                               tttACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGttt                                                                     <  1:169051/137‑1 (MQ=255)
                                                                     atgatCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGaaa                                                             <  1:781419/139‑1 (MQ=255)
                                                                     atgatCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGaaa                                                             <  1:348100/139‑1 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAgg                                                         >  2:257556/1‑139 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTAtt                                                                                  <  2:664682/114‑1 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTAtt                                                                                  >  1:664682/1‑114 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTTTAAACCGTTTGTGTGAAACAgg                                                         >  2:470601/1‑139 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAgg                                                         >  1:617308/1‑139 (MQ=255)
                                                                         tCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTAACGGGTATTTTTTAAACCGTTTGTGTGAAACAgg                                                         >  1:126194/1‑139 (MQ=255)
                                                                           ccGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAggg                                                        <  2:554708/138‑1 (MQ=255)
                                                                           ccGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAggg                                                        <  2:22749/138‑1 (MQ=255)
                                                                           ccGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAggg                                                        <  1:681713/138‑1 (MQ=255)
                                                                                          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccg                                           <  2:181936/136‑1 (MQ=255)
                                                                                          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                         <  1:37576/138‑1 (MQ=255)
                                                                                          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccg                                           <  2:63987/136‑1 (MQ=255)
                                                                                          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgcc                                            <  2:729417/135‑1 (MQ=255)
                                                                                          ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgcc                                            <  1:588363/135‑1 (MQ=255)
                                                                                           tcatcaTGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                         <  1:709883/137‑1 (MQ=255)
                                                                                           tcatcaTGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                         >  2:709883/1‑137 (MQ=255)
                                                                                                         gCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTg                          <  2:385108/138‑1 (MQ=255)
                                                                                                         gCCGTTTGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTg                          <  2:772811/138‑1 (MQ=255)
                                                                                                             tttGTGGCGCGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTAACGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTTTTCCATCTTGCATGt                     >  1:430085/1‑139 (MQ=255)
                                                                                                                 tgGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCat                  <  1:255481/138‑1 (MQ=255)
                                                                                                                   gcgcGACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCattat               <  1:470601/139‑1 (MQ=255)
                                                                                                                       gACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAgggg                                                       >  1:25933/1‑95 (MQ=255)
                                                                                                                       gACGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAgggg                                                       <  2:25933/95‑1 (MQ=255)
                                                                                                                         cGGCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCt         <  1:13329/139‑1 (MQ=255)
                                                                                                                          ggCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt        <  2:846453/139‑1 (MQ=255)
                                                                                                                          ggCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt        <  1:114416/139‑1 (MQ=255)
                                                                                                                          ggCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt        <  1:422003/139‑1 (MQ=255)
                                                                                                                          ggCGATGTGGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt        <  2:124360/139‑1 (MQ=255)
                                                                                                                                 tgGGCCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGtat  <  2:430085/138‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ATGCGCACCGCCCTGCGCACACGCAAAATCACCAATATGCAGTTTGGTGCTTTAACCAGCCTGTTTACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTAT  >  NZ_CP009273/2525413‑2525679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑