Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,815,6920AG63.8% 37.3 / 67.5 58E76G (GAA→GGA) rpoZDNA‑directed RNA polymerase subunit omega
Reads supporting (aligned to +/- strand):  ref base A (10/11);  new base G (19/18);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAT  >  NZ_CP009273/3815558‑3815818
                                                                                                                                      |                                                                                                                              
ggTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGcc                                                                                                                            >  1:160389/1‑139 (MQ=255)
ggTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGcc                                                                                                                            >  2:627888/1‑139 (MQ=255)
ggTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGcc                                                                                                                            >  2:549531/1‑139 (MQ=255)
ggTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGcc                                                                                                                            >  2:473431/1‑139 (MQ=255)
 gTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCg                                                                                                                           <  1:603646/139‑1 (MQ=255)
    ggCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCTAGGAACAGCAAGAGCAGGAAGCCGCTg                                                                                                                        <  1:304789/139‑1 (MQ=255)
    ggCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTg                                                                                                                        <  1:69413/139‑1 (MQ=255)
    ggCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCt                                                                                                                         <  1:602635/138‑1 (MQ=255)
     gCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTg                                                                                                                        >  2:235811/1‑138 (MQ=255)
     gCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTg                                                                                                                        >  2:778878/1‑138 (MQ=255)
     gCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTg                                                                                                                        >  1:759986/1‑138 (MQ=255)
           aGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAGCCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACa                                                                                                                 <  1:278290/139‑1 (MQ=255)
             gATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAg                                                                                                               <  2:332093/139‑1 (MQ=255)
             gATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAg                                                                                                               <  2:759986/139‑1 (MQ=255)
                ccGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCg                                                                                                            >  1:156991/1‑139 (MQ=255)
                  gCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGtt                                                                                                          <  2:117225/139‑1 (MQ=255)
                       tACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGc                                                                                                     >  1:425011/1‑139 (MQ=255)
                         ccGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTa                                                                                                   <  1:778878/139‑1 (MQ=255)
                                  aaCGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAg                                                                                          >  2:240577/1‑139 (MQ=255)
                                      aTAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGgtcg                                                                                      <  2:599053/139‑1 (MQ=255)
                                       tAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGgtcgt                                                                                     >  1:41235/1‑139 (MQ=255)
                                                    aTCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACaaa                                                                        <  2:351155/139‑1 (MQ=255)
                                                    aTCGCGCTGCGCGAAATCAAAGAAGGTCTGAGCAACAACCAGATCCTCGACTTTCGCAAACGCCAGGAACACCAACAGCAGGGAGCCGCTGAATTACAAGCCTTTACCGCTATTGCTGAAGGTCGTCGTTAATCACaaa                                                                        <  1:549531/139‑1 (MQ=255)
                                                       gcgcTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTAc                                                                                                        <  2:746249/104‑1 (MQ=255)
                                                       gcgcTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTAc                                                                                                        >  1:746249/1‑104 (MQ=255)
                                                          cTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGt                                                                  <  1:289859/139‑1 (MQ=255)
                                                            gcgcGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTa                                                                                                   >  2:792549/1‑104 (MQ=255)
                                                            gcgcGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTa                                                                                                   <  1:792549/104‑1 (MQ=255)
                                                             cgcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTc                                                                 <  2:822356/137‑1 (MQ=255)
                                                             cgcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTc                                                                 <  2:463923/137‑1 (MQ=255)
                                                             cgcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTc                                                                 <  1:350461/137‑1 (MQ=255)
                                                             cgcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCg                                                                <  2:425011/138‑1 (MQ=255)
                                                             cgcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGc                                                               >  1:378263/1‑139 (MQ=255)
                                                              gcgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGcc                                                              >  1:705048/1‑139 (MQ=255)
                                                               cgAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGccc                                                             <  2:762122/139‑1 (MQ=255)
                                                                       aagaagGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCt                                                     >  1:567361/1‑139 (MQ=255)
                                                                            ggTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTg                                                <  1:235811/139‑1 (MQ=255)
                                                                                       caacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTg                                                >  2:688229/1‑128 (MQ=255)
                                                                                       caacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTg                                                <  1:688229/128‑1 (MQ=255)
                                                                                       caacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAAt                                     >  2:266456/1‑139 (MQ=255)
                                                                                        aacCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAAt                                     >  1:266987/1‑138 (MQ=255)
                                                                                                ccTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGAtt                            <  2:597836/139‑1 (MQ=255)
                                                                                                   cGACGTTCGCGAACGCCAGGAACAGAAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCa                          >  1:93570/1‑138 (MQ=255)
                                                                                                    gACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCaaa                        >  1:527931/1‑139 (MQ=255)
                                                                                                     aCGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGa                                                                                            >  1:634773/1‑70 (MQ=255)
                                                                                                     aCGTTCGCGAACGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGa                                                                                            <  2:634773/70‑1 (MQ=255)
                                                                                                           gcgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAacctacc                  <  2:705048/138‑1 (MQ=255)
                                                                                                           gcgAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAacctacc                  <  1:399990/138‑1 (MQ=255)
                                                                                                                cGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCgg            <  2:527931/139‑1 (MQ=255)
                                                                                                                cGCCAGGAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCg             >  1:648912/1‑138 (MQ=255)
                                                                                                                     ggAACAGCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGAc       <  1:240577/139‑1 (MQ=255)
                                                                                                                           gCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAt  >  2:522324/1‑138 (MQ=255)
                                                                                                                           gCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAt  >  1:319485/1‑138 (MQ=255)
                                                                                                                           gCAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAt  >  1:21401/1‑138 (MQ=255)
                                                                                                                           gCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAt  >  2:169300/1‑138 (MQ=255)
                                                                                                                           gCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAt  >  1:392554/1‑138 (MQ=255)
                                                                                                                            cAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAAc                                 <  2:566182/106‑1 (MQ=255)
                                                                                                                            cAAGAGCAGGGAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAAc                                 >  1:566182/1‑106 (MQ=255)
                                                                                                                                      |                                                                                                                              
GGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAAT  >  NZ_CP009273/3815558‑3815818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑