Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,435,3790AG63.9% 57.7 / 66.2 61D484G (GAC→GGC) tamBautotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand):  ref base A (11/11);  new base G (18/21);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCG  >  NZ_CP009273/4435244‑4435510
                                                                                                                                       |                                                                                                                                   
aaCAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGt                                                                                                                                  >  2:185888/1‑139 (MQ=255)
   aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               <  1:185888/139‑1 (MQ=255)
   aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               <  2:479866/139‑1 (MQ=255)
   aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               <  2:695977/139‑1 (MQ=255)
   aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               <  1:368616/139‑1 (MQ=255)
   aGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGcc                                                                                                                               <  1:455052/139‑1 (MQ=255)
     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               <  1:191344/137‑1 (MQ=255)
     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGcc                                                                                                                               >  2:191344/1‑137 (MQ=255)
     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCg                                                                                                                              >  2:3292/1‑138 (MQ=255)
     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCg                                                                                                                              >  2:629403/1‑138 (MQ=255)
     gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCg                                                                                                                              >  2:460879/1‑138 (MQ=255)
                    ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCg                                                                                                              >  2:752701/1‑139 (MQ=255)
                                  tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTaaa                                                                                                   >  2:96980/1‑136 (MQ=255)
                                  tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTaaa                                                                                                   >  2:108764/1‑136 (MQ=255)
                                         cttAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAg                                                                                         >  1:415884/2‑139 (MQ=255)
                                         aTTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAg                                                                                         >  2:736203/1‑139 (MQ=255)
                                           tAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGt                                                                                        >  2:488349/1‑138 (MQ=255)
                                            aaCACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTg                                                                                       <  1:604343/138‑1 (MQ=255)
                                            aaCACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTg                                                                                       <  2:842243/138‑1 (MQ=255)
                                                 cGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGtt                                                                                 >  1:631549/1‑139 (MQ=255)
                                                  gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGtt                                                                                 >  1:104171/1‑138 (MQ=255)
                                                  gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGtt                                                                                 >  1:388617/1‑138 (MQ=255)
                                                      aaGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACgg                                                                            <  1:509853/139‑1 (MQ=255)
                                                      aaGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACgg                                                                            >  1:124839/1‑139 (MQ=255)
                                                       agagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGc                                                                           <  2:388617/139‑1 (MQ=255)
                                                        gagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCa                                                                          <  1:359330/139‑1 (MQ=255)
                                                        gagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCa                                                                          <  1:290952/139‑1 (MQ=255)
                                                             cccGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCt                                                                      >  2:6109/1‑138 (MQ=255)
                                                              ccGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGa                                                                    <  1:324609/139‑1 (MQ=255)
                                                                   cTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                               <  1:652899/139‑1 (MQ=255)
                                                                   cTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                               <  2:104171/139‑1 (MQ=255)
                                                                     ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             >  2:226807/1‑139 (MQ=255)
                                                                      gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  1:752701/138‑1 (MQ=255)
                                                                      gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  2:314712/138‑1 (MQ=255)
                                                                      gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  2:773123/138‑1 (MQ=255)
                                                                      gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  2:124839/138‑1 (MQ=255)
                                                                      gCCGTCAAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  1:108764/138‑1 (MQ=255)
                                                                       ccGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                           <  1:6109/139‑1 (MQ=255)
                                                                        cGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAg                                                          >  2:542635/1‑139 (MQ=255)
                                                                                           gATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATc                                       >  1:531070/1‑139 (MQ=255)
                                                                                           gATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATc                                       >  1:674322/1‑139 (MQ=255)
                                                                                             ttAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATccc                                     <  1:3292/139‑1 (MQ=255)
                                                                                             ttAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATccc                                     <  2:552776/139‑1 (MQ=255)
                                                                                               aaaaCCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCa                                    <  1:96980/138‑1 (MQ=255)
                                                                                                  aCCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                           <  1:312034/128‑1 (MQ=255)
                                                                                                  aCCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                           >  2:312034/1‑128 (MQ=255)
                                                                                                       cgGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                           >  1:701387/1‑139 (MQ=255)
                                                                                                       cgGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                           >  1:320025/1‑139 (MQ=255)
                                                                                                       cgGTAGCCTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                           >  1:446430/1‑139 (MQ=255)
                                                                                                              cTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc                    <  1:488349/139‑1 (MQ=255)
                                                                                                              cTGTACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc                    <  1:629403/139‑1 (MQ=255)
                                                                                                                gTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTc                  >  2:329477/1‑139 (MQ=255)
                                                                                                                 tACGGCGGCACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTc                  <  1:226807/138‑1 (MQ=255)
                                                                                                                      cggcACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                           >  2:120527/1‑108 (MQ=255)
                                                                                                                      cggcACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                           <  1:120527/108‑1 (MQ=255)
                                                                                                                      cggcACCTGGCAGATGGGCGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGcc             >  1:213230/1‑138 (MQ=255)
                                                                                                                      cggcACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGcc             >  2:317860/1‑138 (MQ=255)
                                                                                                                        gcACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             <  2:734034/88‑1 (MQ=255)
                                                                                                                        gcACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                             >  1:734034/1‑88 (MQ=255)
                                                                                                                        gcACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa          <  1:460879/139‑1 (MQ=255)
                                                                                                                                cAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCg  <  2:480876/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAACAGTGCCG  >  NZ_CP009273/4435244‑4435510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑