Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	193,289	A→G	H86R (CAC→CGC)	rseP →	sigma E protease regulator RseP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	193,289	0	A	G	100.0%	123.9 / NA	34	H86R (CAC→CGC)	rseP	sigma E protease regulator RseP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

AATAGGGTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCACCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTACGTCCGGTGG  >  NZ_CP009273/193155‑193423                                                                                                                                       |                                                                                                                                       aaTAGGGTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCAt                                                                                                                                    <  2:363974/139‑1 (MQ=255)     gggTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGcc                                                                                                                                 <  1:18345/138‑1 (MQ=255)      ggTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCtt                                                                                                                               >  1:191418/1‑139 (MQ=255)       gTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTc                                                                                                                              <  2:691896/139‑1 (MQ=255)           ggTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCgccgcc                                                                                                                                      <  1:430479/127‑1 (MQ=255)           ggTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCgccgcc                                                                                                                                      >  2:430479/1‑127 (MQ=255)                      ctGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTc                                                                                                               >  1:608648/1‑139 (MQ=255)                       tGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTc                                                                                                                              <  2:236883/123‑1 (MQ=255)                       tGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGGCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTc                                                                                                                              >  1:236883/1‑123 (MQ=255)                         gcggcgAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCgg                                                                                                             <  1:204510/138‑1 (MQ=255)                           ggcgAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGcc                                                                                                           >  1:556620/1‑138 (MQ=255)                               aaCTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAAc                                                                                                        >  2:249612/1‑137 (MQ=255)                                            ggCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTg                                                                                         >  1:694896/1‑139 (MQ=255)                                              cACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGat                                                                                              <  2:580770/132‑1 (MQ=255)                                              cACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGat                                                                                              >  1:580770/1‑132 (MQ=255)                                                   aaTATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAgg                                                                                  >  1:839549/1‑139 (MQ=255)                                                        gTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCgg                                                                             >  1:477516/1‑139 (MQ=255)                                                           aTCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTg                                                                          >  1:828544/1‑139 (MQ=255)                                                             cGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGc                                                                         >  1:111982/1‑138 (MQ=255)                                                             cGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGc                                                                         >  1:380812/1‑138 (MQ=255)                                                                          ttGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           <  1:93245/139‑1 (MQ=255)                                                                          ttGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           <  2:362373/139‑1 (MQ=255)                                                                          ttGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           <  2:608648/139‑1 (MQ=255)                                                                          ttGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           <  1:249612/139‑1 (MQ=255)                                                                             ggCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTa                                                        <  2:698369/139‑1 (MQ=255)                                                                             ggCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTa                                                        <  2:191418/139‑1 (MQ=255)                                                                             ggCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTa                                                        <  2:650077/139‑1 (MQ=255)                                                                                                   aTGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGtttt                                  <  1:318266/139‑1 (MQ=255)                                                                                                             aGAACCGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGgt                        <  1:450794/139‑1 (MQ=255)                                                                                                                  cGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGcc                   <  2:491754/139‑1 (MQ=255)                                                                                                                  cGGTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGcc                   <  1:203483/139‑1 (MQ=255)                                                                                                                    gTCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTg                 >  2:539155/1‑139 (MQ=255)                                                                                                                     tCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           <  2:816956/96‑1 (MQ=255)                                                                                                                     tCGTTCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTg                                                           >  1:816956/1‑96 (MQ=255)                                                                                                                        ttCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGt             <  2:839549/139‑1 (MQ=255)                                                                                                                         tCCGGAACTCCGCCGCCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTa            >  2:456309/1‑139 (MQ=255)                                                                                                                                   cgccgcCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTACGTCCggtgg  >  1:532875/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       AATAGGGTTTGGTAAGGCGCTCTGGCGGCGAACTGATAAGCTCGGCACCGAATATGTTATCGCCCTGATCCCGTTGGGCGGTTATGTCAAAATGCTGGATGAGCGCGCAGAACCGGTCGTTCCGGAACTCCGCCACCATGCCTTCAATAATAAATCTGTCGGCCAACGAGCGGCGATTATTGCCGCAGGTCCGGTTGCAAACTTCATTTTTGCTATCTTTGCCTACTGGCTGGTTTTTATTATTGGTGTGCCTGGCGTACGTCCGGTGG  >  NZ_CP009273/193155‑193423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑