Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,595,866	A→G	I81T (ATT→ACT)	ftsE ←	cell division ATP‑binding protein FtsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,595,866	0	A	G	100.0%	252.7 / NA	65	I81T (ATT→ACT)	ftsE	cell division ATP‑binding protein FtsE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/28);  total (37/28)

CCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAAT  >  NZ_CP009273/3595730‑3596001                                                                                                                                         |                                                                                                                                        cccGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTc                                                                                                                                       >  1:153908/1‑139 (MQ=255)           tCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGgcggcg                                                                                                                               >  1:292869/1‑137 (MQ=255)                cgccgcCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAAc                                                                                                                        >  1:763788/1‑139 (MQ=255)                cgccgcCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAAc                                                                                                                        >  1:37603/1‑139 (MQ=255)                          acCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCAc                                                                                                              >  2:343427/1‑139 (MQ=255)                          acCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCAc                                                                                                              <  2:499867/139‑1 (MQ=255)                                      atatCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGAc                                                                                                  <  1:515714/139‑1 (MQ=255)                                           gTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTg                                                                                             <  1:343427/139‑1 (MQ=255)                                                          ggCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCAc                                                                                                              >  2:396183/1‑107 (MQ=255)                                                          ggCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCAc                                                                                                              <  1:396183/107‑1 (MQ=255)                                                          ggCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCt                                                                               >  2:509783/1‑138 (MQ=255)                                                             gATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                                           <  2:292869/139‑1 (MQ=255)                                                                  tCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGAt                                                                      <  2:701291/139‑1 (MQ=255)                                                                      cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGgcgc                                                                                                                              <  2:147104/79‑1 (MQ=255)                                                                      cGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGgcgc                                                                                                                              >  1:147104/1‑79 (MQ=255)                                                                       gggATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 <  1:304914/139‑1 (MQ=255)                                                                                        gTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCa                                                <  2:99730/139‑1 (MQ=255)                                                                                         tAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTccc                                                                <  1:537964/122‑1 (MQ=255)                                                                                         tAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTccc                                                                >  2:537964/1‑122 (MQ=255)                                                                                         tAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCaa                                               <  1:713267/139‑1 (MQ=255)                                                                                              aGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          <  1:571625/139‑1 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCa                                                                                                     >  2:502459/1‑78 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCa                                                                                                     <  1:502459/78‑1 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTg                                                                                             <  1:686242/86‑1 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTg                                                                                             >  2:686242/1‑86 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                                           >  2:729290/1‑104 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                                           <  1:729290/104‑1 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCa                                                >  1:579604/1‑131 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCa                                                <  2:579604/131‑1 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  1:181972/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  2:51786/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  2:531891/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  2:619690/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  1:430029/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  1:713483/1‑137 (MQ=255)                                                                                                tACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCca                                          >  1:192609/1‑137 (MQ=255)                                                                                                     tCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAGCTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGc                                                                                >  1:315935/1‑94 (MQ=255)                                                                                                     tCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAGCTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGc                                                                                <  2:315935/94‑1 (MQ=255)                                                                                                     tCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 >  2:303460/1‑109 (MQ=255)                                                                                                     tCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 <  1:303460/109‑1 (MQ=255)                                                                                                        aTCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCt                                >  2:465730/1‑139 (MQ=255)                                                                                                        aTCAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCt                                >  1:731453/1‑139 (MQ=255)                                                                                                          cAGTAGATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCtt                               >  2:826217/1‑138 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAg                                                                                                    >  1:27899/1‑64 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAg                                                                                                    <  2:27899/64‑1 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                                           >  1:273796/1‑89 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAAc                                                                           <  2:273796/89‑1 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggag                         >  1:483924/1‑139 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCaggag                         >  1:603921/1‑139 (MQ=255)                                                                                                               gATGGTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCagga                          <  2:430029/138‑1 (MQ=255)                                                                                                                   gTGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTa                     >  2:818635/1‑139 (MQ=255)                                                                                                                    tGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGAt                                                                      >  1:7082/1‑89 (MQ=255)                                                                                                                    tGATCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGAt                                                                      <  2:7082/89‑1 (MQ=255)                                                                                                                      aTCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGa                                      <  1:21802/119‑1 (MQ=255)                                                                                                                      aTCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGa                                      >  2:21802/1‑119 (MQ=255)                                                                                                                       tCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 >  1:713039/1‑91 (MQ=255)                                                                                                                       tCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 <  2:713039/91‑1 (MQ=255)                                                                                                                       tCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCtt                               >  1:816352/1‑125 (MQ=255)                                                                                                                       tCCTGGAAAATCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCtt                               <  2:816352/125‑1 (MQ=255)                                                                                                                             aaaaTCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 <  1:426496/85‑1 (MQ=255)                                                                                                                             aaaaTCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTcc                                                                 >  2:426496/1‑85 (MQ=255)                                                                                                                             aaaaTCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTcc              <  2:605569/136‑1 (MQ=255)                                                                                                                             aaaaTCATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTcc              >  1:605569/1‑136 (MQ=255)                                                                                                                                  cATGCCAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCg      >  2:548173/1‑139 (MQ=255)                                                                                                                                      ccAGTCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAAt  <  2:713483/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAAT  >  NZ_CP009273/3595730‑3596001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑