Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,750,235	T→C	E318G (GAA→GGA)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,750,235	0	T	C	100.0%	188.5 / NA	52	E318G (GAA→GGA)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/31);  total (21/31)

GCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGG  >  NZ_CP009273/3750099‑3750366                                                                                                                                         |                                                                                                                                    gCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTccc                                                                                                                                   >  1:118278/1‑139 (MQ=255)  ccACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcg                                                                                                                                  >  1:815506/1‑139 (MQ=255)  ccACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcg                                                                                                                                  >  1:392996/1‑139 (MQ=255)    acaGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  2:815506/139‑1 (MQ=255)    acaGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  1:286531/139‑1 (MQ=255)    acaGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  2:265619/139‑1 (MQ=255)      aGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGcgtc                                                                                                                              >  2:298583/1‑139 (MQ=255)           tCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGtttt                                                                                                                         >  2:365300/1‑139 (MQ=255)               tCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTAc                                                                                                                      <  1:440420/138‑1 (MQ=255)                         aGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGtt                                                                                                            <  2:185130/138‑1 (MQ=255)                          gTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTc                                                                                                           <  2:118278/138‑1 (MQ=255)                             gCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGTGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACg                                                                                                        <  2:597513/138‑1 (MQ=255)                              ccAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGcc                                                                                                      <  2:94280/139‑1 (MQ=255)                                  tACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCAtt                                                                                                  >  1:315306/1‑139 (MQ=255)                                      gcgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAg                                                                                              <  1:569682/139‑1 (MQ=255)                                      gcgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAg                                                                                              <  1:641832/139‑1 (MQ=255)                                       cgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGc                                                                                             >  2:85162/1‑139 (MQ=255)                                           aTCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGca                                                                                         <  1:54065/139‑1 (MQ=255)                                           aTCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGca                                                                                         <  2:354259/139‑1 (MQ=255)                                                   cttTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCa                                                                                   <  1:304680/137‑1 (MQ=255)                                                      tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  2:848074/1‑136 (MQ=255)                                                                gTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAgcg                                                                    >  1:381931/1‑139 (MQ=255)                                                                    cgTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGa                                                                >  2:27291/1‑139 (MQ=255)                                                                      tAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACt                                                              <  2:381931/139‑1 (MQ=255)                                                                          ccTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGc                                                          <  1:250709/139‑1 (MQ=255)                                                                              ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAg                                                                                              >  2:698454/1‑99 (MQ=255)                                                                              ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAg                                                                                              <  1:698454/99‑1 (MQ=255)                                                                                gggATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGa                                                    <  1:298583/139‑1 (MQ=255)                                                                                   atatCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTg                                                                                                 >  2:729519/1‑91 (MQ=255)                                                                                   atatCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTg                                                                                                 <  1:729519/91‑1 (MQ=255)                                                                                                tcGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCgg                                    >  1:313140/1‑139 (MQ=255)                                                                                                 cGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCgg                                    <  2:581660/138‑1 (MQ=255)                                                                                                       ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                >  2:616531/1‑40 (MQ=255)                                                                                                       ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  1:616531/40‑1 (MQ=255)                                                                                                       ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCaa                              >  2:546516/1‑138 (MQ=255)                                                                                                       ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCaa                              >  1:106487/1‑138 (MQ=255)                                                                                                       ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCaa                              >  1:760949/1‑138 (MQ=255)                                                                                                            ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTTAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:697747/139‑1 (MQ=255)                                                                                                            ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:458741/139‑1 (MQ=255)                                                                                                            ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:848074/139‑1 (MQ=255)                                                                                                            ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:106487/139‑1 (MQ=255)                                                                                                            ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:312052/139‑1 (MQ=255)                                                                                                             gATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCg                       <  1:27291/139‑1 (MQ=255)                                                                                                              aTGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGt                      <  2:586662/139‑1 (MQ=255)                                                                                                                       aTGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTtacataca              <  2:760949/138‑1 (MQ=255)                                                                                                                            ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  1:82232/66‑1 (MQ=255)                                                                                                                            ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  2:82232/1‑66 (MQ=255)                                                                                                                            ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        >  1:389018/1‑123 (MQ=255)                                                                                                                            ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:389018/123‑1 (MQ=255)                                                                                                                            ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCgtgt        >  1:727811/1‑139 (MQ=255)                                                                                                                                 cAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      <  2:633760/88‑1 (MQ=255)                                                                                                                                 cAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      >  1:633760/1‑88 (MQ=255)                                                                                                                                  aGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAgg  >  1:566813/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    GCCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGG  >  NZ_CP009273/3750099‑3750366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑