Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,781,002 A→G T269A (ACG→GCG)  envC → murein hydrolase activator EnvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,781,0020AG98.4% 208.1 / ‑3.7 62T269A (ACG→GCG) envCmurein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (25/36);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01

GCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAA  >  NZ_CP009273/3780866‑3781133
                                                                                                                                        |                                                                                                                                   
gCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAgcggcg                                                                                                                                   <  1:222683/139‑1 (MQ=255)
       ttGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAg                                                                                                                            >  2:636482/1‑139 (MQ=255)
       ttGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGAGCGCAAAg                                                                                                                            >  1:420142/1‑139 (MQ=255)
           gcgggcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:790377/139‑1 (MQ=255)
           gcgagcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:304150/139‑1 (MQ=255)
           gcgagcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:194493/139‑1 (MQ=255)
           gcgagcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:139949/139‑1 (MQ=255)
              agcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcaccac                                                                                                                     >  2:98095/1‑139 (MQ=255)
               gcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  1:258089/135‑1 (MQ=255)
               gcTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        >  2:258089/1‑135 (MQ=255)
                 tGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTa                                                                                                                  >  1:69934/1‑139 (MQ=255)
                   cgcgcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:249485/131‑1 (MQ=255)
                   cgcgcCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAACGGCGCGCAAAGGcac                                                                                                                        >  1:249485/1‑131 (MQ=255)
                                    tCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGa                                                                                                >  1:599662/1‑138 (MQ=255)
                                        cGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAAt                                                                                           >  1:801026/1‑139 (MQ=255)
                                           aaCAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  2:326055/107‑1 (MQ=255)
                                           aaCAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        >  1:326055/1‑107 (MQ=255)
                                             cAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTg                                                                                      <  1:807343/139‑1 (MQ=255)
                                             cAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTg                                                                                      <  2:453637/139‑1 (MQ=255)
                                                cATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATg                                                                                   >  1:593361/1‑139 (MQ=255)
                                                  ttGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTc                                                                                 >  2:470521/1‑139 (MQ=255)
                                                  ttGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTc                                                                                 >  2:597937/1‑139 (MQ=255)
                                                     cccGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCg                                                                              >  2:348576/1‑139 (MQ=255)
                                                        gTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        >  2:316934/1‑94 (MQ=255)
                                                        gTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGcac                                                                                                                        <  1:316934/94‑1 (MQ=255)
                                                        gTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAAACGCTGAAGTCCCGTAc                                                                           >  1:787325/1‑139 (MQ=255)
                                                         tGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTAcc                                                                          <  2:69934/139‑1 (MQ=255)
                                                          gCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCg                                                                         >  1:32209/1‑139 (MQ=255)
                                                           cGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCgg                                                                        <  1:359987/139‑1 (MQ=255)
                                                           cGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCg                                                                         <  1:296662/138‑1 (MQ=255)
                                                                      gcgAAAGCGCGTGCAGAACGAGAAGCGCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCGCCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGTTGGTCTGGgcg                                                             <  2:115964/139‑1 (MQ=255)
                                                                        gAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGgcgcg                                                           <  1:224345/139‑1 (MQ=255)
                                                                           aGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGcgccg                                                        >  2:455535/1‑139 (MQ=255)
                                                                             cgcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCcgcg                                                      <  1:636482/139‑1 (MQ=255)
                                                                               cgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCaa                                                                                                                              <  1:157699/65‑1 (MQ=255)
                                                                               cgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCaa                                                                                                                              >  2:157699/1‑65 (MQ=255)
                                                                                 tGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGCCCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGGGGTCTGGGCGCGCCGCGGGGt                                                  >  2:757021/1‑139 (MQ=255)
                                                                                    aGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAg                                               <  1:597937/139‑1 (MQ=255)
                                                                                             aGCACGTGAGGCCCAGGCGGTTCTCCACCGCAAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTg                                                                                      >  2:571473/1‑91 (MQ=255)
                                                                                             aGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTgg                                      >  2:321338/1‑139 (MQ=255)
                                                                                             aGCACGTGAGGCCCAGGCGGTTCGCGACCGCAAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTg                                                                                      <  1:571473/91‑1 (MQ=255)
                                                                                                aCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCcgcg                                                      <  2:42193/120‑1 (MQ=255)
                                                                                                aCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCcgcg                                                      >  1:42193/1‑120 (MQ=255)
                                                                                                 cGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCgg                                  >  1:99486/1‑139 (MQ=255)
                                                                                                          cAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc                         <  1:321338/139‑1 (MQ=255)
                                                                                                          cAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc                         <  1:348247/139‑1 (MQ=255)
                                                                                                          cAGGCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc                         <  1:507825/139‑1 (MQ=255)
                                                                                                             gCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGa                      <  2:436549/139‑1 (MQ=255)
                                                                                                             gCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGa                      <  1:633527/139‑1 (MQ=255)
                                                                                                             gCGGTTCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGa                      <  1:403537/139‑1 (MQ=255)
                                                                                                                 ttCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTc                                                                                 <  2:484298/76‑1 (MQ=255)
                                                                                                                 ttCGCGACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTc                                                                                 >  1:484298/1‑76 (MQ=255)
                                                                                                                   cgcgACCGCCAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTg                 <  2:279166/138‑1 (MQ=255)
                                                                                                                   cgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTg                 <  2:790046/138‑1 (MQ=255)
                                                                                                                           ccAGAAAGAAGCGGTGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTAt        <  2:68327/139‑1 (MQ=255)
                                                                                                                           ccAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc                         <  2:361923/122‑1 (MQ=255)
                                                                                                                           ccAGAAAGAAGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGc                         >  1:361923/1‑122 (MQ=255)
                                                                                                                               aaagaaGCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCg    <  1:706436/139‑1 (MQ=255)
                                                                                                                                 agaagCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGaa  <  1:470521/139‑1 (MQ=255)
                                                                                                                                 agaagCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGaa  <  2:544800/139‑1 (MQ=255)
                                                                                                                                 agaagCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGaa  <  1:98095/139‑1 (MQ=255)
                                                                                                                                 agaagCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGaa  <  2:128162/139‑1 (MQ=255)
                                                                                                                                 agaagCGGCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGaa  <  2:99486/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GCAACAGTTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAA  >  NZ_CP009273/3780866‑3781133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑