BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,805,353	T→C	E189E (GAA→GAG)	radC ←	DNA repair protein RadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,805,353	0	T	C	100.0%	130.6 / NA	35	E189E (GAA→GAG)	radC	DNA repair protein RadC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/14); total (21/14)

ACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAG  >  NZ_CP009273/3805220‑3805487

aCAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCCAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAg                                                                                                                                    >  2:245631/1‑138 (MQ=255)

aCAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAg                                                                                                                                    >  1:152144/1‑138 (MQ=255)

aCAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAg                                                                                                                                    >  1:547773/1‑138 (MQ=255)

          cGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTa                                                                                                                         >  1:421771/1‑139 (MQ=255)

           gAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTAt                                                                                                                        <  1:233958/139‑1 (MQ=255)

            aaGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATc                                                                                                                       >  2:786871/1‑139 (MQ=255)

              ggATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCg                                                                                                                     <  2:768719/139‑1 (MQ=255)

                    cgcATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTAc                                                                                                               >  1:515506/1‑139 (MQ=255)

                     gcATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACt                                                                                                              <  2:152144/139‑1 (MQ=255)

                           cGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGtt                                                                                                         >  1:235353/1‑138 (MQ=255)

                              gTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGc                                                                                                     <  2:567689/139‑1 (MQ=255)

                                        gCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCg                                                                                             >  1:495375/1‑137 (MQ=255)

                                        gCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGaa                                                                                           <  2:642577/139‑1 (MQ=255)

                                           gcgTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAggg                                                                                        <  1:548828/139‑1 (MQ=255)

                                                 ggCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGAtt                                                                                   <  1:640690/138‑1 (MQ=255)

                                                       aGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCa                                                                            <  2:776435/139‑1 (MQ=255)

                                                                 tctcCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAgcg                                                                  >  2:18025/1‑139 (MQ=255)

                                                                   tcCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGcc                                                                >  1:224629/1‑139 (MQ=255)

                                                                     cACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGcc                                                                >  2:906/1‑137 (MQ=255)

                                                                      aCGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAg                                                             <  2:224629/139‑1 (MQ=255)

                                                                      aCGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAg                                                             <  2:235353/139‑1 (MQ=255)

                                                                                    tatGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTAt                                               >  2:370556/1‑139 (MQ=255)

                                                                                         tCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCtt                                          >  2:177150/1‑139 (MQ=255)

                                                                                          cGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTc                                         >  1:205780/1‑139 (MQ=255)

                                                                                            aGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTcgc                                       >  2:740453/1‑139 (MQ=255)

                                                                                              cACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTcgcg                                      >  2:820304/1‑138 (MQ=255)

                                                                                                   gTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCTGAGGCGTTTATTTTTATCGCTTCGCGGATAAt                                <  1:740453/139‑1 (MQ=255)

                                                                                                   gTAAATCCATGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAAt                                <  2:495375/139‑1 (MQ=255)

                                                                                                           aTGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAg                        >  1:486250/1‑139 (MQ=255)

                                                                                                            tGAACTGACAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAg                        >  1:295096/1‑138 (MQ=255)

                                                                                                                   aCAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGcac                                                                                                   <  2:402106/56‑1 (MQ=255)

                                                                                                                   aCAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGcac                                                                                                   >  1:402106/1‑56 (MQ=255)

                                                                                                                   aCAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGAc                 <  1:786871/138‑1 (MQ=255)

                                                                                                                   aCAACTCTTTATTATCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACt                >  2:111521/1‑139 (MQ=255)

                                                                                                                                 tCCGCTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAg  >  2:315019/1‑139 (MQ=255)

ACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAG  >  NZ_CP009273/3805220‑3805487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`