Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,816,198	C→T	L147L (CTG→TTG)	spoT →	bifunctional GTP diphosphokinase/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,816,198	0	C	T	100.0%	253.1 / NA	64	L147L (CTG→TTG)	spoT	bifunctional GTP diphosphokinase/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/30);  total (34/30)

AACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTG  >  NZ_CP009273/3816064‑3816329                                                                                                                                       |                                                                                                                                    aaCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 <  1:91250/139‑1 (MQ=255) aaCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 <  2:497561/139‑1 (MQ=255)  aCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGc                                                                                                                                >  1:40945/1‑139 (MQ=255)       aTAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACt                                                                                                                           >  1:563441/1‑139 (MQ=255)       aTAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACt                                                                                                                           >  2:416272/1‑139 (MQ=255)                 gTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGa                                                                                                                  >  2:73869/1‑138 (MQ=255)                     cgcgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAAc                                                                                                             >  2:842717/1‑139 (MQ=255)                      gcgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACg                                                                                                            >  2:722644/1‑139 (MQ=255)                       cgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGt                                                                                                           >  1:756993/1‑139 (MQ=255)                       cgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGt                                                                                                           >  2:323801/1‑139 (MQ=255)                              aaagaGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCa                                                                                                    <  2:266987/139‑1 (MQ=255)                                    gcgcAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGccc                                                                                              >  1:485726/1‑139 (MQ=255)                                        aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTg                                                                                           >  1:475863/1‑138 (MQ=255)                                              aaaaCTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAActct                                                                                    >  2:623356/1‑139 (MQ=255)                                                           gatgatTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGc                                                                       <  2:475863/139‑1 (MQ=255)                                                                     gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:40945/138‑1 (MQ=255)                                                                     gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:377695/138‑1 (MQ=255)                                                                      cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAGCATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:338044/139‑1 (MQ=255)                                                                      cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:563441/139‑1 (MQ=255)                                                                      cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  1:499306/139‑1 (MQ=255)                                                                      cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  1:623356/139‑1 (MQ=255)                                                                        aTGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  1:591777/137‑1 (MQ=255)                                                                        aTGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            >  2:591777/1‑137 (MQ=255)                                                                          ggTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGc                                                                                                                       <  1:767110/76‑1 (MQ=255)                                                                          ggTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGc                                                                                                                       >  2:767110/1‑76 (MQ=255)                                                                              cAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:266602/129‑1 (MQ=255)                                                                              cAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              >  2:266602/1‑129 (MQ=255)                                                                              cAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAg                                                    <  2:353060/139‑1 (MQ=255)                                                                                     tCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 <  2:148663/55‑1 (MQ=255)                                                                                     tCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 >  1:148663/1‑55 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 >  2:201468/1‑54 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 <  1:201468/54‑1 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCAc                                                           >  1:98804/1‑124 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCAc                                                           <  2:98804/124‑1 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGtt                                                       >  1:795985/1‑128 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGtt                                                       <  2:795985/128‑1 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:779106/1‑139 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:355137/1‑139 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:712916/1‑139 (MQ=255)                                                                                      ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:567187/1‑139 (MQ=255)                                                                                        gcgTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccacca                                          <  2:540907/139‑1 (MQ=255)                                                                                           tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            >  1:482207/1‑118 (MQ=255)                                                                                           tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:482207/118‑1 (MQ=255)                                                                                           tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  2:598301/1‑139 (MQ=255)                                                                                           tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  1:555074/1‑139 (MQ=255)                                                                                            cATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  1:578504/1‑138 (MQ=255)                                                                                             aTCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAtt                                      >  2:756747/1‑138 (MQ=255)                                                                                                  catcaAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTa                                     >  1:276623/1‑134 (MQ=255)                                                                                                  catcaAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTa                                     <  2:276623/134‑1 (MQ=255)                                                                                                         cTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCg                         <  1:73869/139‑1 (MQ=255)                                                                                                             ccGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGa                                                                                 <  1:49829/79‑1 (MQ=255)                                                                                                             ccGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGa                                                                                 >  2:49829/1‑79 (MQ=255)                                                                                                                          caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAActc                                                                                     >  2:655384/1‑62 (MQ=255)                                                                                                                          caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAActc                                                                                     <  1:655384/62‑1 (MQ=255)                                                                                                                          caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg        <  2:567187/139‑1 (MQ=255)                                                                                                                           aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCGTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAAc                                 <  1:63971/113‑1 (MQ=255)                                                                                                                           aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAgg       >  2:608299/1‑139 (MQ=255)                                                                                                                           aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAgg       <  1:722644/139‑1 (MQ=255)                                                                                                                           aacaTGCGCACGTGGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAAc                                 >  2:63971/1‑113 (MQ=255)                                                                                                                              aTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc    <  1:598301/139‑1 (MQ=255)                                                                                                                              aTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc    >  2:853796/1‑139 (MQ=255)                                                                                                                                gcgcACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:294910/79‑1 (MQ=255)                                                                                                                                gcgcACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              >  1:294910/1‑79 (MQ=255)                                                                                                                                 cgcACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTg  <  2:779106/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    AACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTG  >  NZ_CP009273/3816064‑3816329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑