Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,913,243 A→C V174G (GTC→GGC)  atpH ← F0F1 ATP synthase subunit delta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,913,2430AC100.0% 155.5 / NA 43V174G (GTC→GGC) atpHF0F1 ATP synthase subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/18);  total (25/18)

GTCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTC  >  NZ_CP009273/3913110‑3913376
                                                                                                                                     |                                                                                                                                     
gTCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCt                                                                                                                                  >  1:607145/1‑139 (MQ=255)
 tCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTg                                                                                                                                 >  2:556558/1‑139 (MQ=255)
 tCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTg                                                                                                                                 >  2:457469/1‑139 (MQ=255)
 tCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTg                                                                                                                                 >  1:410236/1‑139 (MQ=255)
       aCAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAg                                                                                                                             <  1:836719/137‑1 (MQ=255)
       aCAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGgc                                                                                                                           >  2:621489/1‑139 (MQ=255)
        cAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGgcg                                                                                                                          <  1:829999/139‑1 (MQ=255)
             aCAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTc                                                                                                                       >  2:179501/1‑137 (MQ=255)
                           gTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACg                                                                                                       <  2:111855/139‑1 (MQ=255)
                           gTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACg                                                                                                       <  1:846099/139‑1 (MQ=255)
                                   cTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATc                                                                                               <  1:206666/139‑1 (MQ=255)
                                             aacaTTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCata                                                                                     >  2:473666/1‑139 (MQ=255)
                                                  tGAACTGAGCAATGCGCTGCTTGATCAGTTCGGTGATTCGAGTGGAATGGAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCAcc                                                                                >  1:577852/1‑139 (MQ=255)
                                                  tGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATg                                                                                           <  2:732019/128‑1 (MQ=255)
                                                  tGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATg                                                                                           >  1:732019/1‑128 (MQ=255)
                                                  tGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCAcc                                                                                >  1:708437/1‑139 (MQ=255)
                                                         aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCg                                                                          >  1:265587/1‑138 (MQ=255)
                                                         aGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCAAGTCCCCTTAAGACTGCAAGCCTTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACAATATCACCCGCTCg                                                                          >  1:675033/1‑138 (MQ=255)
                                                              tGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGc                                                                                                   >  2:16830/1‑108 (MQ=255)
                                                              tGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGc                                                                                                   <  1:16830/108‑1 (MQ=255)
                                                                     ctTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCt                                                             >  1:241678/1‑139 (MQ=255)
                                                                        gATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                                                            <  1:179501/137‑1 (MQ=255)
                                                                               tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTa                                                      >  2:563252/1‑136 (MQ=255)
                                                                               tCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTa                                                      >  1:276810/1‑136 (MQ=255)
                                                                                     aTTTCGGTGGAATTCATTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTAt                                             <  2:241678/139‑1 (MQ=255)
                                                                                     aTTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTAt                                             >  2:764024/1‑139 (MQ=255)
                                                                                        tCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGa                                          >  2:39153/1‑139 (MQ=255)
                                                                                         cGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGa                                          <  2:817174/138‑1 (MQ=255)
                                                                                         cGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGa                                          <  2:265587/138‑1 (MQ=255)
                                                                                                aTTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCaa                                  <  1:621489/139‑1 (MQ=255)
                                                                                                   cAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAAtt                                <  1:748547/138‑1 (MQ=255)
                                                                                                      ttGCATGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGc                            >  2:343825/1‑139 (MQ=255)
                                                                                                           tGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGgcg                                                                                                                          >  2:131541/1‑40 (MQ=255)
                                                                                                           tGCTCCAGTCCCCTTAAGACTGCAAGCCGTCTGCAAGgcg                                                                                                                          <  1:131541/40‑1 (MQ=255)
                                                                                                                       cTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCt                                                                            <  2:436566/74‑1 (MQ=255)
                                                                                                                       cTTAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCt                                                                            >  1:436566/1‑74 (MQ=255)
                                                                                                                        ttAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                                                            >  2:472538/1‑89 (MQ=255)
                                                                                                                        ttAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                                                            <  1:472538/89‑1 (MQ=255)
                                                                                                                        ttAAGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGa          >  1:279595/1‑139 (MQ=255)
                                                                                                                          aaGACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGa          <  2:607145/137‑1 (MQ=255)
                                                                                                                            gACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                                                            >  2:476940/1‑85 (MQ=255)
                                                                                                                            gACTGCAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTg                                                            <  1:476940/85‑1 (MQ=255)
                                                                                                                                 cAAGCCGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTc  >  1:571392/1‑138 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GTCACTTACAGAAACAATAGTACCTTCGTTGTGAGCTTCACTCACAACATTGAACTGAGCAATGCGCTGCTTGATCAGTTCGCTGATTTCGGTGGAATTCAGTTGCATGCTCCAGTCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTC  >  NZ_CP009273/3913110‑3913376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑