Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,994,324	A→G	V31V (GTT→GTC)	yigE ←	phosphodiester glycosidase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,994,324	0	A	G	100.0%	154.4 / NA	43	V31V (GTT→GTC)	yigE	phosphodiester glycosidase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/17);  total (26/17)

GTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAG  >  NZ_CP009273/3994205‑3994453                                                                                                                        |                                                                                                                                  gTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTa                                                                                                                <  2:752205/139‑1 (MQ=255)     cccACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGt                                                                                                            <  1:670231/139‑1 (MQ=255)       cACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGt                                                                                                            >  2:19768/1‑137 (MQ=255)              cGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAg                                                                                                                  >  2:676826/1‑124 (MQ=255)              cGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAg                                                                                                                  <  1:676826/124‑1 (MQ=255)              cGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGc                                                                                                      >  1:118992/1‑136 (MQ=255)                      gCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATcc                                                                                           >  2:410343/1‑139 (MQ=255)                                  tACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTc                                                                                >  2:722020/1‑138 (MQ=255)                                              cGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCTTTTTAGATTCAAGGTGATCATTc                                                                   <  1:797876/139‑1 (MQ=255)                                               gCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTcc                                                                  >  2:223586/1‑139 (MQ=255)                                                     tttGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTa                                                                                                                <  2:485100/87‑1 (MQ=255)                                                     tttGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTa                                                                                                                >  1:485100/1‑87 (MQ=255)                                                       tGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCa                                                           <  2:265132/138‑1 (MQ=255)                                                         aGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAAt                                                         >  1:621219/1‑138 (MQ=255)                                                           gATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAg                                                      >  1:492155/1‑139 (MQ=255)                                                               aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAg                                                      >  1:657432/1‑135 (MQ=255)                                                               aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAg                                                      <  2:657432/135‑1 (MQ=255)                                                               aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGc                                                  >  2:321671/1‑139 (MQ=255)                                                               aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGc                                                  >  2:287927/1‑139 (MQ=255)                                                               aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGc                                                  >  2:248347/1‑139 (MQ=255)                                                                    tataCGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATg                                             <  1:223586/139‑1 (MQ=255)                                                                      taCGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg                                           <  2:235169/139‑1 (MQ=255)                                                                            tGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                     >  2:454032/1‑139 (MQ=255)                                                                            tGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                     >  2:315820/1‑139 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCTAGAAAAATCCGTTTGAGATTCAAGGTGATCTTTCCTTTACCAATGAGTAGCTGATg                                             <  1:966/129‑1 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAg                                                                             >  2:458240/1‑97 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAg                                                                             <  1:458240/97‑1 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATg                                             >  2:966/1‑129 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                     >  1:88466/1‑137 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                     >  1:629104/1‑137 (MQ=255)                                                                              tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                     >  1:308736/1‑137 (MQ=255)                                                                                   tCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTcc                              >  1:276088/1‑139 (MQ=255)                                                                                     aGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCt                             >  2:310623/1‑138 (MQ=255)                                                                                     aGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCt                             <  1:310623/138‑1 (MQ=255)                                                                                        gTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                                                                                       <  1:511648/77‑1 (MQ=255)                                                                                        gTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                                                                                       >  2:511648/1‑77 (MQ=255)                                                                                                  agagTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGa                   <  2:118992/135‑1 (MQ=255)                                                                                                        cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGa                                               >  1:669800/1‑101 (MQ=255)                                                                                                        cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGa                                               <  2:669800/101‑1 (MQ=255)                                                                                                        cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc         >  1:850914/1‑139 (MQ=255)                                                                                                        cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc         <  1:454032/139‑1 (MQ=255)                                                                                                        cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc         >  1:332299/1‑139 (MQ=255)                                                                                                               atcaGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAg  <  2:308736/139‑1 (MQ=255)                                                                                                                   gcggcgACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                                                                                       >  1:10238/1‑50 (MQ=255)                                                                                                                   gcggcgACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGttt                                                                                       <  2:10238/50‑1 (MQ=255)                                                                                                                        |                                                                                                                                  GTTCCCCACGCTTCGCCATTGGCTTTTTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAG  >  NZ_CP009273/3994205‑3994453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑