Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,041,206	G→A	G294D (GGC→GAC)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,041,206	0	G	A	100.0%	146.1 / NA	36	G294D (GGC→GAC)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

TGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGGCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAACA  >  NZ_CP009273/4041071‑4041339                                                                                                                                        |                                                                                                                                      tGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACa                                                                                                                                     >  1:426829/1‑138 (MQ=255)   gCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAAt                                                                                                                                  >  1:442479/1‑139 (MQ=255)                   aCGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCaa                                                                                                                     >  1:545531/1‑136 (MQ=255)                   aCGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCaa                                                                                                                     <  2:545531/136‑1 (MQ=255)                   aCGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAgg                                                                                                                  >  1:241780/1‑139 (MQ=255)                   aCGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAgg                                                                                                                  >  2:552544/1‑139 (MQ=255)                           cTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAAc                                                                                                          <  2:382875/139‑1 (MQ=255)                                    aCCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCcgcg                                                                                                  <  2:241780/138‑1 (MQ=255)                                    aCCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGa                                                                                                 <  2:356758/139‑1 (MQ=255)                                                   gAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAAc                                                                                    >  1:499892/1‑137 (MQ=255)                                                    aaGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAg                                                                                 <  1:297226/139‑1 (MQ=255)                                                         cagcaACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTg                                                                            <  2:774039/139‑1 (MQ=255)                                                              aCCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGc                                                                                              >  1:468432/1‑116 (MQ=255)                                                              aCCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGc                                                                                              <  2:468432/116‑1 (MQ=255)                                                              aCCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGAc                                                                       >  1:300291/1‑139 (MQ=255)                                                                cGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGa                                                                     <  1:195146/139‑1 (MQ=255)                                                                 ggCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACg                                                                      >  2:625152/1‑137 (MQ=255)                                                                        gAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAg                                                             <  2:368202/139‑1 (MQ=255)                                                                                      gCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACg                                               >  1:53855/1‑139 (MQ=255)                                                                                         gTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGt                                             >  2:158377/1‑138 (MQ=255)                                                                                         gTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGt                                             >  2:473555/1‑138 (MQ=255)                                                                                          ttCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGa                                           <  1:552544/139‑1 (MQ=255)                                                                                          ttCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGa                                           >  1:65567/1‑139 (MQ=255)                                                                                                  gTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTAt                                   <  2:53855/139‑1 (MQ=255)                                                                                                    aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGATACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGa                                 >  1:685990/1‑139 (MQ=255)                                                                                                     tGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGAc                                <  2:754657/139‑1 (MQ=255)                                                                                                     tGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGAc                                <  1:153703/139‑1 (MQ=255)                                                                                                             aaCGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGt                        >  1:181471/1‑139 (MQ=255)                                                                                                                   ggACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCAt                  >  1:269071/1‑139 (MQ=255)                                                                                                                         ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGc                                                                                              >  2:471215/1‑57 (MQ=255)                                                                                                                         ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGc                                                                                              <  1:471215/57‑1 (MQ=255)                                                                                                                         ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTtga            >  1:416989/1‑139 (MQ=255)                                                                                                                         ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTtga            >  2:839329/1‑139 (MQ=255)                                                                                                                              gTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAg                                                                   >  2:101607/1‑79 (MQ=255)                                                                                                                              gTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAg                                                                   <  1:101607/79‑1 (MQ=255)                                                                                                                                   aGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAaca  >  2:239078/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      TGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGGCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAACA  >  NZ_CP009273/4041071‑4041339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑