Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,062,410	G→T	P76Q (CCG→CAG)	yihQ ←	sulfoquinovosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,062,410	0	G	T	100.0%	132.3 / NA	34	P76Q (CCG→CAG)	yihQ	sulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/14);  total (20/14)

GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGG  >  NZ_CP009273/4062277‑4062545                                                                                                                                      |                                                                                                                                        gcgcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGAc                                                                                                                                    <  2:570485/139‑1 (MQ=255)                 ttGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGt                                                                                                                    >  2:172033/1‑139 (MQ=255)                               gTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAtt                                                                                                      >  2:375906/1‑139 (MQ=255)                                tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAttt                                                                                                     >  2:343868/1‑139 (MQ=255)                                      tGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCATCCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTcc                                                                                                <  2:489348/138‑1 (MQ=255)                                      tGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTcc                                                                                                >  2:364939/1‑138 (MQ=255)                                      tGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATAGTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTcc                                                                                                <  1:364939/138‑1 (MQ=255)                                           ttCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGt                                                                                          >  1:155987/1‑139 (MQ=255)                                                  aaTAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCtt                                                                                   <  2:199066/139‑1 (MQ=255)                                                      aaCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAAt                                                                               >  1:423073/1‑139 (MQ=255)                                                       aCGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATg                                                                              >  2:775886/1‑139 (MQ=255)                                                            ccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGa                                                                          >  2:655253/1‑138 (MQ=255)                                                                aTCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTa                                                                     >  1:716313/1‑139 (MQ=255)                                                                     cgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                                >  1:501870/1‑139 (MQ=255)                                                                     cgGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                                >  2:648040/1‑139 (MQ=255)                                                                      ggCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                                <  1:655253/138‑1 (MQ=255)                                                                                  cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                                    <  2:155987/138‑1 (MQ=255)                                                                                   aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                                   <  1:101246/138‑1 (MQ=255)                                                                                   aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                                   <  2:716313/138‑1 (MQ=255)                                                                                       tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGc                                              >  2:257152/1‑139 (MQ=255)                                                                                        ggCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGc                                              >  2:494904/1‑138 (MQ=255)                                                                                         gcgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                                <  2:274629/119‑1 (MQ=255)                                                                                         gcgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                                >  1:274629/1‑119 (MQ=255)                                                                                          cgcTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCta                                            >  2:578967/1‑138 (MQ=255)                                                                                            cTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAc                                         <  2:501870/139‑1 (MQ=255)                                                                                                    aGAACCACGGCTGAAATGAATTAACCATCCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaaa                                                                        <  1:786069/100‑1 (MQ=255)                                                                                                    aGAACCACGGCTGAAATGAATTAACCATCCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGaaa                                                                        >  2:786069/1‑100 (MQ=255)                                                                                                      aaCCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAAt                               >  1:625491/1‑139 (MQ=255)                                                                                                           cGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCAt                           >  1:43374/1‑138 (MQ=255)                                                                                                                               accaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTa      <  1:455934/139‑1 (MQ=255)                                                                                                                               accaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTa      <  1:775886/139‑1 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAGCATATCGATATCCGCTATACCTGAGCCAATCCATAAAc                       <  2:293064/121‑1 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAAc                       >  1:293064/1‑121 (MQ=255)                                                                                                                                   tCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATggg  >  1:2639/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATCTTTGCTATGGG  >  NZ_CP009273/4062277‑4062545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑