Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,078,866	C→T	S106N (AGC→AAC)	fdnG ←	formate dehydrogenase‑N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,078,866	0	C	T	100.0%	130.7 / NA	33	S106N (AGC→AAC)	fdnG	formate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAC  >  NZ_CP009273/4078740‑4079000                                                                                                                               |                                                                                                                                       tGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGa                                                                                                                            >  1:415909/1‑139 (MQ=255)  gcgcAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGa                                                                                                                            <  1:342584/138‑1 (MQ=255)      aaTGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaaa                                                                                                                       <  2:171801/139‑1 (MQ=255)      aaTGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaaa                                                                                                                       <  1:357890/139‑1 (MQ=255)                                tcatcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCgg                                                                                                <  2:415909/136‑1 (MQ=255)                                   tcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAg                                                                                          >  2:270069/1‑139 (MQ=255)                                       tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                      >  2:720470/1‑139 (MQ=255)                                       tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                      >  2:399985/1‑139 (MQ=255)                                                 cgatCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGa                                                                            >  1:202309/1‑139 (MQ=255)                                                 cgatCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGa                                                                            >  2:629187/1‑139 (MQ=255)                                                   atCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGAcc                                                                          <  2:146968/139‑1 (MQ=255)                                                              ctTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCg                                                                >  2:679905/1‑138 (MQ=255)                                                                 cccAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  1:68326/137‑1 (MQ=255)                                                                 cccAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGAt                                                             <  2:202309/138‑1 (MQ=255)                                                                    aaCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGcc                                                         <  1:629187/139‑1 (MQ=255)                                                                      cTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCt                                                        >  2:439450/1‑138 (MQ=255)                                                                         aTTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  2:428294/129‑1 (MQ=255)                                                                         aTTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              >  1:428294/1‑129 (MQ=255)                                                                         aTTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCt                                                        >  2:646859/1‑135 (MQ=255)                                                                            tgctgcCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  <  1:646859/138‑1 (MQ=255)                                                                                                 ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAag                                            >  2:351853/1‑123 (MQ=255)                                                                                                 ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAag                                            <  1:351853/123‑1 (MQ=255)                                                                                                 ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                            >  2:823725/1‑139 (MQ=255)                                                                                                 ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                            >  1:587764/1‑139 (MQ=255)                                                                                                 ggCGCACGGTATTCCGGAAACTTCAAACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                            >  2:613219/1‑139 (MQ=255)                                                                                                  gcgcACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGtt                           >  1:782626/1‑139 (MQ=255)                                                                                                                  aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGAt                                                             >  2:55503/1‑89 (MQ=255)                                                                                                                  aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGAt                                                             <  1:55503/89‑1 (MQ=255)                                                                                                                  aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCg           <  1:328998/139‑1 (MQ=255)                                                                                                                    aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  2:695119/1‑139 (MQ=255)                                                                                                                      ttCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       >  1:211752/1‑139 (MQ=255)                                                                                                                        cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  1:695119/137‑1 (MQ=255)                                                                                                                           aCGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAc  <  2:782626/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                       TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAC  >  NZ_CP009273/4078740‑4079000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑