Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,078,948	C→A	E79* (GAA→TAA)	fdnG ←	formate dehydrogenase‑N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,078,948	0	C	A	100.0%	158.3 / NA	41	E79* (GAA→TAA)	fdnG	formate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/23);  total (18/23)

TGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTT  >  NZ_CP009273/4078815‑4079068                                                                                                                                      |                                                                                                                         tgctgcCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                                                                                      <  1:646859/138‑1 (MQ=255)                      ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAag                                                                                                                <  1:351853/123‑1 (MQ=255)                      ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAag                                                                                                                >  2:351853/1‑123 (MQ=255)                      ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                                                                                                >  1:587764/1‑139 (MQ=255)                      ggCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                                                                                                >  2:823725/1‑139 (MQ=255)                      ggCGCACGGTATTCCGGAAACTTCAAACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGt                                                                                                >  2:613219/1‑139 (MQ=255)                       gcgcACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGtt                                                                                               >  1:782626/1‑139 (MQ=255)                                       aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCg                                                                               <  1:328998/139‑1 (MQ=255)                                         aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg                                                                             >  2:695119/1‑139 (MQ=255)                                           ttCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc                                                                           >  1:211752/1‑139 (MQ=255)                                             cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc                                                                           <  1:695119/137‑1 (MQ=255)                                                aCGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAc                                                                      <  2:782626/139‑1 (MQ=255)                                                     tttCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCaa                                                                 >  1:31090/1‑139 (MQ=255)                                                      ttCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCaa                                                                 <  1:399985/138‑1 (MQ=255)                                                       tCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAAc                                                                <  2:608456/138‑1 (MQ=255)                                                       tCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAAc                                                                <  2:184577/138‑1 (MQ=255)                                                            gTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCca                                                          <  1:270069/139‑1 (MQ=255)                                                             tGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCcac                                                         <  1:439450/139‑1 (MQ=255)                                                               gATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCAc                                                                                 <  2:293163/113‑1 (MQ=255)                                                               gATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCAc                                                                                 >  1:293163/1‑113 (MQ=255)                                                                aTGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACa                                                               <  1:544902/130‑1 (MQ=255)                                                                aTGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACa                                                               >  2:544902/1‑130 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGc                                                                                                  <  2:588433/81‑1 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGc                                                                                                  >  1:588433/1‑81 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCcaca                                                        >  2:487325/1‑123 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCcaca                                                        <  1:487325/123‑1 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCG‑TTTTTGCTCCGTCAc                                                                                 <  2:26268/97‑1 (MQ=255)                                                                              gCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCG‑TTTTTGCTCCGTCAc                                                                                 >  1:26268/1‑97 (MQ=255)                                                                                  gcgcCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTg                                     >  1:643281/1‑138 (MQ=255)                                                                                   cgcCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCaa                                                                 <  2:716402/109‑1 (MQ=255)                                                                                   cgcCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCaa                                                                 >  1:716402/1‑109 (MQ=255)                                                                                   cgcCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCa                                   <  2:31090/139‑1 (MQ=255)                                                                                    gcCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCGCCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAg                                  <  1:823725/139‑1 (MQ=255)                                                                                            gACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACg                          <  2:587764/139‑1 (MQ=255)                                                                                                  gTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTc                    >  2:451347/1‑139 (MQ=255)                                                                                                          cgGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTgc            >  2:37162/1‑139 (MQ=255)                                                                                                           ggTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACACTAGGTGCAGGTATTACGGGTTTCGCGGGTgcg           <  1:749516/139‑1 (MQ=255)                                                                                                           ggTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTgcg           <  1:243738/139‑1 (MQ=255)                                                                                                             ttGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCgca         <  2:230349/139‑1 (MQ=255)                                                                                                             ttGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCgca         <  1:37162/139‑1 (MQ=255)                                                                                                                    ggTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGGGCAGGGATTACGGGTTTCGCGGGTGCGCAGCAGttt  >  2:83002/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                         TGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTT  >  NZ_CP009273/4078815‑4079068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑