Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,222,007	A→C	G385G (GGT→GGG)	lysC ←	lysine‑sensitive aspartokinase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,222,007	0	A	C	100.0%	141.5 / NA	39	G385G (GGT→GGG)	lysC	lysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/20);  total (19/20)

CAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGAT  >  NZ_CP009273/4221872‑4222138                                                                                                                                        |                                                                                                                                    cAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCct                                                                                                                                  <  2:120930/139‑1 (MQ=255)           aGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTtccacct                                                                                                                        <  1:851197/139‑1 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTtccacct                                                                                                                        >  1:692707/1‑127 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTtccacct                                                                                                                        <  2:692707/127‑1 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGaca                                                                                                              >  2:103919/1‑137 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGaca                                                                                                              >  1:342277/1‑137 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGaca                                                                                                              >  1:516094/1‑137 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGAcaca                                                                                                            <  2:782647/139‑1 (MQ=255)                       gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGAcaca                                                                                                            <  1:643584/139‑1 (MQ=255)                             ccATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGccc                                                                                                                                   <  2:778234/110‑1 (MQ=255)                             ccATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGccc                                                                                                                                   >  1:778234/1‑110 (MQ=255)                                      aTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCa                                                                                             <  1:283495/139‑1 (MQ=255)                                            cGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcag                                                                                         >  1:49053/1‑137 (MQ=255)                                                     aaCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGc                                                                                                 <  2:344419/120‑1 (MQ=255)                                                     aaCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGc                                                                                                 >  1:344419/1‑120 (MQ=255)                                                          ttCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                                                                         <  2:697724/139‑1 (MQ=255)                                                            ccAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCaa                                                                       >  2:355472/1‑139 (MQ=255)                                                                  cGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTAt                                                                  <  2:516094/138‑1 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAgaga                                                                                   >  2:697365/1‑119 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAgaga                                                                                   <  1:697365/119‑1 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                                                                         >  2:278562/1‑129 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                                                                         <  1:278562/129‑1 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc                                                               >  1:717224/1‑139 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc                                                               >  2:645940/1‑139 (MQ=255)                                                                    ccGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc                                                               >  1:468745/1‑139 (MQ=255)                                                                        aTACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGt                                                           <  2:837007/139‑1 (MQ=255)                                                                               tttGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGtt                                                    >  2:230070/1‑139 (MQ=255)                                                                                 tGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTg                                                   >  2:658506/1‑138 (MQ=255)                                                                                   ccAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAAc                                                <  2:49053/139‑1 (MQ=255)                                                                                       cgccgcAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCggt                                            <  1:432079/139‑1 (MQ=255)                                                                                       cgccgcAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCggt                                            >  1:16907/1‑139 (MQ=255)                                                                                                          gTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc                                                               <  2:594678/101‑1 (MQ=255)                                                                                                          gTCATTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGc                                                               >  1:594678/1‑101 (MQ=255)                                                                                                             aTTGCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACg                      >  1:511114/1‑139 (MQ=255)                                                                                                                gCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCt                    <  2:468745/138‑1 (MQ=255)                                                                                                                gCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCt                    <  1:754367/138‑1 (MQ=255)                                                                                                                gCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCt                    <  1:385145/138‑1 (MQ=255)                                                                                                                gCCAATCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCt                    <  1:230070/138‑1 (MQ=255)                                                                                                                    aTCAACGCGACCAGCGCCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACtt               >  1:853339/1‑139 (MQ=255)                                                                                                                          gcgACCAGCGCCAGCTCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACg         <  1:645940/139‑1 (MQ=255)                                                                                                                                  cgcCAGCCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGAt  >  2:793038/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                    CAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGAT  >  NZ_CP009273/4221872‑4222138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑