Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,469,838	C→T	L98F (CTT→TTT)	yjgN →	YjgN family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,469,838	0	C	T	100.0%	129.7 / NA	35	L98F (CTT→TTT)	yjgN	YjgN family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

ATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCT  >  NZ_CP009273/4469704‑4469973                                                                                                                                       |                                                                                                                                        aTCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTttgt                                                                                                                                     >  1:160456/1‑139 (MQ=255) aTCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTttgt                                                                                                                                     >  2:410738/1‑139 (MQ=255) aTCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTttgt                                                                                                                                     >  2:227595/1‑139 (MQ=255)      tATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggtt                                                                                                                                >  2:170235/1‑139 (MQ=255)                   aaGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACttt                                                                                                                   >  2:535933/1‑139 (MQ=255)                     gTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGt                                                                                                                 >  1:814998/1‑139 (MQ=255)                                aCGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGtt                                                                                                      >  2:262639/1‑139 (MQ=255)                                aCGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGtt                                                                                                      <  2:165476/139‑1 (MQ=255)                                       tCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                                <  1:410738/138‑1 (MQ=255)                                       tCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                                <  1:706687/138‑1 (MQ=255)                                       tCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                                <  1:793906/138‑1 (MQ=255)                                                 ttACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGgc                                                                                     <  2:760223/139‑1 (MQ=255)                                                              tGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGgc                                                                                     >  2:813228/1‑126 (MQ=255)                                                              tGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGgc                                                                                     <  1:813228/126‑1 (MQ=255)                                                               gTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGc                                                                       >  2:802066/1‑139 (MQ=255)                                                                tttttGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGc                                                                       <  1:582843/138‑1 (MQ=255)                                                                      ttAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGtcatc                                                                 <  1:172622/138‑1 (MQ=255)                                                                        aGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAgg                                                              >  2:207725/1‑139 (MQ=255)                                                                                  tttttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGa                                                     <  1:262639/138‑1 (MQ=255)                                                                                    tttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                   <  2:597655/138‑1 (MQ=255)                                                                                    tttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                   <  2:188471/138‑1 (MQ=255)                                                                                                        aaTCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGAttt                              <  1:802066/139‑1 (MQ=255)                                                                                                                  aCAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAAt                     <  1:207725/138‑1 (MQ=255)                                                                                                                  aCAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAAt                     <  2:130749/138‑1 (MQ=255)                                                                                                                    aGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGc                  >  1:195602/1‑139 (MQ=255)                                                                                                                    aGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGc                  <  2:734865/139‑1 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCaa                                           <  2:740823/112‑1 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCaa                                           >  1:740823/1‑112 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc                >  1:158622/1‑139 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc                >  2:776572/1‑139 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc                >  1:273155/1‑139 (MQ=255)                                                                                                                      tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc                >  2:816391/1‑139 (MQ=255)                                                                                                                              gATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAg        >  2:563051/1‑139 (MQ=255)                                                                                                                                tatGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAggg      >  2:187404/1‑139 (MQ=255)                                                                                                                                    ccGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCt  >  2:391506/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCT  >  NZ_CP009273/4469704‑4469973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑