| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,516,576 | A→G | Y44H (TAT→CAT) | sgcR ← | DeoR/GlpR family DNA‑binding transcription regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,516,576 | 0 | A | G | 97.6% | 152.2 / ‑3.8 | 42 | Y44H (TAT→CAT) | sgcR | DeoR/GlpR family DNA‑binding transcription regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (22/18); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
TCTCTTTTCGCCACAGATTGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCT > NZ_CP009273/4516452‑4516702 | tctcTTTTCGCCACAGATTGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCacgac > 2:276681/1‑139 (MQ=255) cGCCACAGATTGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGt < 1:840436/138‑1 (MQ=255) gATTGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCg < 2:382184/139‑1 (MQ=255) tGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCa > 2:558170/1‑138 (MQ=255) tACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCaaa < 2:571820/139‑1 (MQ=255) gTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAg > 1:481544/1‑139 (MQ=255) ttCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCt > 1:300777/1‑139 (MQ=255) gTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATg > 2:800934/1‑139 (MQ=255) gTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATg > 2:371451/1‑139 (MQ=255) gAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGc < 2:459453/138‑1 (MQ=255) tACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCa < 2:556889/111‑1 (MQ=255) tACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCa > 1:556889/1‑111 (MQ=255) ctctTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCtgtgtg > 1:270333/1‑139 (MQ=255) tGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGga < 1:53490/136‑1 (MQ=255) tGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATCCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGGGGagag > 1:345897/1‑139 (MQ=255) tcTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGAc > 1:668798/1‑139 (MQ=255) cTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGAc > 1:381860/1‑138 (MQ=255) cTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGAc > 2:737111/1‑138 (MQ=255) aCTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATg < 2:643357/138‑1 (MQ=255) tcgtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTgg > 2:276843/1‑63 (MQ=255) tcgtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTgg < 1:276843/63‑1 (MQ=255) tcgtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGcc < 1:558170/139‑1 (MQ=255) gtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCa > 1:534934/1‑88 (MQ=255) gtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCa < 2:534934/88‑1 (MQ=255) gtcgAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCca > 2:653849/1‑138 (MQ=255) gAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAgg > 2:293989/1‑139 (MQ=255) ccAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCctg < 1:568655/103‑1 (MQ=255) ccAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCctg > 2:568655/1‑103 (MQ=255) ccAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTg > 2:585284/1‑139 (MQ=255) aaCCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCAt < 2:426658/139‑1 (MQ=255) aaCCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCAt < 1:729098/139‑1 (MQ=255) aTCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGt < 2:345897/139‑1 (MQ=255) aTCATGCCTGGATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGt < 2:300777/139‑1 (MQ=255) gATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCctg < 2:658883/73‑1 (MQ=255) gATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCctg > 1:658883/1‑73 (MQ=255) gATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGcc < 1:696693/99‑1 (MQ=255) gATACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGcc > 2:696693/1‑99 (MQ=255) aTACTGGTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGt > 2:708111/1‑139 (MQ=255) ggTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCt > 2:689978/1‑139 (MQ=255) gTTGACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCt > 1:129352/1‑138 (MQ=255) gACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATg > 1:433309/1‑84 (MQ=255) gACAATATGCTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATg < 2:433309/84‑1 (MQ=255) | TCTCTTTTCGCCACAGATTGCAATGTACGTTTCACGTCGAAGACGTACTCTTTGTCGTCTGTACTGTCGTCGAAGTCCAGGCAACCGTGACCGCGGATCATGCCTGGATACTGGTTGACAATATACTGGAAATCACGACGGACAGTCGCTTCCGCATAGCCAAAAAGCTCCATGGCCTGCTGTGTGGAGAGGTGACGATGTTGCCACAGGTAGTGCAGCATCTGTTTGATACGGTCAGGTCTCTGTTGGCT > NZ_CP009273/4516452‑4516702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |