Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,770	T→C	E145G (GAA→GGA)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,770	0	T	C	100.0%	174.0 / NA	48	E145G (GAA→GGA)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/22);  total (26/22)

GGTTGGAGGTGAAGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCATC  >  NZ_CP009273/251636‑251903                                                                                                                                       |                                                                                                                                      ggTTGGAGGTGAAGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGt                                                                                                                                   >  2:758514/1‑139 (MQ=255)     ggAGGTGAAGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATg                                                                                                                               >  2:488911/1‑139 (MQ=255)       aGGTGAAGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGt                                                                                                                             <  2:421569/139‑1 (MQ=255)            aaGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATg                                                                                                                               >  1:353972/1‑132 (MQ=255)            aaGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATg                                                                                                                               <  2:353972/132‑1 (MQ=255)                   tACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTc                                                                                                                 >  1:673514/1‑139 (MQ=255)                         ccGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCgg                                                                                                           >  1:149426/1‑139 (MQ=255)                                aCCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTg                                                                                                     >  1:565869/1‑138 (MQ=255)                                    aTGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCa                                                                                                >  1:486592/1‑139 (MQ=255)                                      gTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACg                                                                                              >  2:671217/1‑139 (MQ=255)                                           tttCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTc                                                                                         >  2:742936/1‑139 (MQ=255)                                            ttCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCg                                                                                        >  1:428849/1‑139 (MQ=255)                                               aCCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGt                                                                                       >  1:455568/1‑137 (MQ=255)                                                    cttcttcGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGc                                                                                   >  2:428464/1‑136 (MQ=255)                                                          cGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGc                                                                          >  1:331752/1‑139 (MQ=255)                                                           ggAGTCGGTGTTAATCAGAATATCCGCATACATCCAGTTCCCCTGTAACCCGAACGCCCCGTCCATCCCGGCTTCTCCGGTCATGGTCAGCAGCACTCCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGcc                                                                         <  1:233695/139‑1 (MQ=255)                                                             aGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAg                                                                       >  1:32617/1‑139 (MQ=255)                                                                 ggTTTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   <  1:428464/139‑1 (MQ=255)                                                                 ggTGTTAATCAGAATATCAGCCTTCACCCAGTTGCCCTGTAACCCGAACGCCCCGTCCATCCCGGCTTCTCCGGTCATGGTCAGCAGCCCTCCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   <  2:208741/139‑1 (MQ=255)                                                                 ggTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTCCCAGCGGGCCGTGAACCACGTTTTCTTCAGCCAGAACCGCCAGCGCa                                                                   <  2:409520/139‑1 (MQ=255)                                                                 ggTGTTAATCAAAATATCAGCCTGCACCCAGTTCCCCTGTAAGCCGACCGCCCCGTCCATACCGGCTTCTCCGGTCATGGTCAGCACCACTTCCAGCGGGCCGTGACCCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   <  2:32617/139‑1 (MQ=255)                                                                 ggTGTTAATCAAAATACCAGCCTGCAACCAGTTGCCCTGTAAGCCGACCGCCCCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTCCCAGCGGGCCGTGAACCACTTTTCCGTCAGCCAGAACCGCCAGCGCa                                                                   <  2:455568/139‑1 (MQ=255)                                                                 ggTGTTAACAAGAATTACAGCCTCCACCCAGTTCCCCTGCAACCCGACCGCCCCGCCCATCCCGGCTTCTCCGGCCATGGTCAGCACCCCTCCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   <  2:486592/139‑1 (MQ=255)                                                                  gtgtTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTcagc                                                                                                                         <  2:715919/84‑1 (MQ=255)                                                                  gtgtTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTcagc                                                                                                                         >  1:715919/1‑84 (MQ=255)                                                                       aaccAGAATATCAGCCGGCAACCAGTTGCCCTGTAAGCCGACCGCCCCGTCCATCCCGGCTTCTCCGGTCATGGTCAGCAGCCCTTCCAGCGGGCCGTGACCCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGc                                                              <  2:673514/135‑1 (MQ=255)                                                                             aaTATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   >  2:80275/1‑127 (MQ=255)                                                                             aaTATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCa                                                                   <  1:80275/127‑1 (MQ=255)                                                                             aaTATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATAcc                                                        >  1:664800/1‑138 (MQ=255)                                                                             aaTATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATAcc                                                        >  2:341890/1‑138 (MQ=255)                                                                             aaTATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATAcc                                                        >  1:504671/1‑138 (MQ=255)                                                                                     ccTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGt                                                >  2:452276/1‑138 (MQ=255)                                                                                         cAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCCTACCAATGCCGTTATcc                                           >  2:555976/1‑139 (MQ=255)                                                                                              aGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCAcc                                      <  1:758514/139‑1 (MQ=255)                                                                                               gTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGt                                                                                                                             >  1:376980/1‑51 (MQ=255)                                                                                               gTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGt                                                                                                                             <  2:376980/51‑1 (MQ=255)                                                                                               gTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCAcc                                      >  2:501199/1‑138 (MQ=255)                                                                                                        tAAGCCGAACGCACCGCCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCgtggtg                            <  1:354757/139‑1 (MQ=255)                                                                                                             cGAACGCACCTGCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTCATCCGCACCCAGCGTGGTGCcgcg                       <  1:362730/139‑1 (MQ=255)                                                                                                             cGAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCcgcg                       <  2:331752/139‑1 (MQ=255)                                                                                                              gAACGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCcgcgc                      >  2:170336/1‑139 (MQ=255)                                                                                                                aCGCACCGTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCgcgcgc                    >  1:212443/1‑139 (MQ=255)                                                                                                                       gTCCATACCGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAAcc             >  1:613566/1‑139 (MQ=255)                                                                                                                               cGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCTAGCGTGGTGCCGCGCGCTTTAACCCATTCGcc     <  1:742936/139‑1 (MQ=255)                                                                                                                               cGGCTTCTCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGcc     <  1:170336/139‑1 (MQ=255)                                                                                                                               cGGCTTCCCCGGTCATGGTCAGCAGCACTCCCGGCGGGCCGTGACCCCCGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGcc     <  2:212443/139‑1 (MQ=255)                                                                                                                                 gCTTCTCCGTTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCAt   <  1:626382/139‑1 (MQ=255)                                                                                                                                 gCTTCCCCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGACCCCCGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCAt   <  2:613566/139‑1 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCaa                                                      <  1:510045/87‑1 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCaa                                                      >  2:510045/1‑87 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCAcc                                      >  2:156054/1‑103 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCAcc                                      <  1:156054/103‑1 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCa                                    >  2:181852/1‑105 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCa                                    <  1:181852/105‑1 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCAt   >  2:626382/1‑138 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCAt   >  2:166575/1‑138 (MQ=255)                                                                                                                                  cttctcCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCAt   >  1:846910/1‑138 (MQ=255)                                                                                                                                   ttctccGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCATc  >  1:30618/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                      GGTTGGAGGTGAAGTCGATACCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCACTTCCAGCGGGCCGTGAACCACGTTTTCGTCAGCCAGAACCGCCAGCGCAGAGGCCATACCAATGCCGTTATCCGCACCCAGCGTGGTGCCGCGCGCTTTAACCCATTCGCCATC  >  NZ_CP009273/251636‑251903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑