Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,616,195	A→G	E156G (GAA→GGA)	radA →	DNA repair protein RadA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,616,195	0	A	G	100.0%	148.7 / NA	40	E156G (GAA→GGA)	radA	DNA repair protein RadA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

CTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACG  >  NZ_CP009273/4616062‑4616328                                                                                                                                      |                                                                                                                                      ctACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAAc                                                                                                                                    >  1:313499/1‑137 (MQ=255)  tACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAg                                                                                                                                 >  2:270194/1‑139 (MQ=255)    cTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCa                                                                                                                               <  2:417225/139‑1 (MQ=255)    cTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCa                                                                                                                               <  2:169642/139‑1 (MQ=255)        aaaCGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGa                                                                                                                           <  2:313499/139‑1 (MQ=255)        aaaCGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGa                                                                                                                           <  2:560892/139‑1 (MQ=255)                tgCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCt                                                                                                                   >  2:704000/1‑139 (MQ=255)                      cTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGa                                                                                                             >  2:820682/1‑139 (MQ=255)                         gCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGAt                                                                                                            <  2:361774/137‑1 (MQ=255)                                   tGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAagaaga                                                                                                <  2:606632/139‑1 (MQ=255)                                       nnngCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAagaaga                                                                                                <  2:64907/132‑1 (MQ=255)                                                        gCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAAt                                                                           >  2:448707/1‑139 (MQ=255)                                                           aaGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGa                                                                        <  2:359788/139‑1 (MQ=255)                                                             gagTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                                      <  1:492084/139‑1 (MQ=255)                                                             gagTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                                      <  1:429341/139‑1 (MQ=255)                                                                   cTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATc                                                                 >  2:637569/1‑138 (MQ=255)                                                                          aGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGAt                                                         >  2:181899/1‑139 (MQ=255)                                                                           ggTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATg                                                        <  1:448707/139‑1 (MQ=255)                                                                                cAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCatat                                                   <  1:780649/139‑1 (MQ=255)                                                                                   tGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGc                                                <  2:578061/139‑1 (MQ=255)                                                                                   tGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGc                                                <  1:40527/139‑1 (MQ=255)                                                                                            aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTAc                                        <  1:181899/138‑1 (MQ=255)                                                                                                ccTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCa                                   <  2:11696/139‑1 (MQ=255)                                                                                                    ggCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                                      >  1:814243/1‑100 (MQ=255)                                                                                                    ggCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                                      <  2:814243/100‑1 (MQ=255)                                                                                                    ggCCTGCCGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGc                               >  2:261353/1‑139 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGa                                                                                                                           >  2:142879/1‑41 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGa                                                                                                                           <  1:142879/41‑1 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGAt                                                         >  2:360941/1‑107 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGAt                                                         <  1:360941/107‑1 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTgg                           <  2:736364/137‑1 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTgg                           >  2:592393/1‑137 (MQ=255)                                                                                                          ccGACTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTgg                           >  1:736364/1‑137 (MQ=255)                                                                                                             aCTGACAATCTCAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCg                      <  1:94314/139‑1 (MQ=255)                                                                                                                       tcAATATGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGt            >  1:734245/1‑139 (MQ=255)                                                                                                                           tatGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGcc                              >  2:822786/1‑117 (MQ=255)                                                                                                                           tatGTTGTCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGcc                              <  1:822786/117‑1 (MQ=255)                                                                                                                               ttgtCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGaaa    >  1:788330/1‑139 (MQ=255)                                                                                                                                tgtCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAc   >  1:536911/1‑139 (MQ=255)                                                                                                                                 gtCGGGAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcg  >  1:178426/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      CTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACG  >  NZ_CP009273/4616062‑4616328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑