Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	345,822	A→T	T34S (ACC→TCC)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	345,822	0	A	T	100.0%	177.5 / NA	44	T34S (ACC→TCC)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/22);  total (22/22)

TCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCG  >  NZ_CP009273/345686‑345954                                                                                                                                         |                                                                                                                                     tctcGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTcc                                                                                                                                    >  2:145930/1‑139 (MQ=255)           aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTaa                                                                                                                            >  1:176713/1‑137 (MQ=255)           aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTaa                                                                                                                            >  2:657318/1‑137 (MQ=255)                  aTAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                    <  1:776273/138‑1 (MQ=255)                    aaCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAAt                                                                                                                  >  2:711528/1‑138 (MQ=255)                         tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTg                                                                                                            >  2:6982/1‑139 (MQ=255)                                gACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATg                                                                                                                 >  1:253575/1‑127 (MQ=255)                                gACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATg                                                                                                                 <  2:253575/127‑1 (MQ=255)                                gACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTAcc                                                                                                     >  1:56950/1‑139 (MQ=255)                                    acaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCgg                                                                                                 <  1:618539/139‑1 (MQ=255)                                       tGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                              <  2:296797/139‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           <  1:288407/139‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           <  1:145930/139‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           <  2:600746/139‑1 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           >  1:686839/1‑139 (MQ=255)                                          gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           <  2:435177/139‑1 (MQ=255)                                           cGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGa                                                                                           >  2:269395/1‑138 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                               >  1:594114/1‑116 (MQ=255)                                             acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                               <  2:594114/116‑1 (MQ=255)                                               acaACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                               <  2:515669/114‑1 (MQ=255)                                               acaACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                               >  1:515669/1‑114 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                    >  1:247221/1‑100 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                    <  2:247221/100‑1 (MQ=255)                                                        cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTgg                                                                             >  1:542182/1‑139 (MQ=255)                                                           cAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCg                                                                           >  1:447570/1‑138 (MQ=255)                                                           cAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCAC‑TTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                    >  2:798229/1‑96 (MQ=255)                                                           cAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCAC‑TTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                    <  1:798229/96‑1 (MQ=255)                                                                   tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                                                  >  1:558566/1‑139 (MQ=255)                                                                          gTCATTAAACCGAAAAAATATGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAttt                                                            >  1:706130/1‑138 (MQ=255)                                                                           tCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTg                                                           <  2:542182/138‑1 (MQ=255)                                                                             aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTg                                                           >  1:836930/1‑136 (MQ=255)                                                                                      aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                               <  1:6982/139‑1 (MQ=255)                                                                                      aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                               <  2:558566/139‑1 (MQ=255)                                                                                      aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                               <  2:314601/139‑1 (MQ=255)                                                                                                tgGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGAt                                     <  2:428746/139‑1 (MQ=255)                                                                                                   cACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCa                                  <  1:218890/139‑1 (MQ=255)                                                                                                     cTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACg                                <  2:706130/139‑1 (MQ=255)                                                                                                                  cggcggGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGTCGCATCTGCTGATCCACGGCAAACTGCCGAc                   <  2:273579/139‑1 (MQ=255)                                                                                                                         cAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                                                            <  1:796161/59‑1 (MQ=255)                                                                                                                         cAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                                                            >  2:796161/1‑59 (MQ=255)                                                                                                                                 cgcTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgc    >  2:7564/1‑139 (MQ=255)                                                                                                                                   ctctGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATCCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgccg  >  1:24274/1‑139 (MQ=255)                                                                                                                                      tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATc                                    <  2:423242/102‑1 (MQ=255)                                                                                                                                      tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATc                                    >  1:423242/1‑102 (MQ=255)                                                                                                                                         |                                                                                                                                     TCTCGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCG  >  NZ_CP009273/345686‑345954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑