Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	443,081	C→T	L27L (CTG→CTA)	cyoE ←	heme o synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	443,081	0	C	T	100.0%	133.5 / NA	36	L27L (CTG→CTA)	cyoE	heme o synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

CGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGT  >  NZ_CP009273/442949‑443201                                                                                                                                      |                                                                                                                          cGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGa                                                                                                                       <  1:628192/137‑1 (MQ=255)  gTCCTATCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAAt                                                                                                                     <  1:713557/138‑1 (MQ=255)        tCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCaa                                                                                                              >  1:273000/1‑139 (MQ=255)         ccATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCaa                                                                                                              <  1:432047/138‑1 (MQ=255)                     cGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAg                                                                                                 <  2:263249/139‑1 (MQ=255)                      gATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAgg                                                                                                <  2:693900/139‑1 (MQ=255)                              tGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCa                                                                                          >  1:73828/1‑137 (MQ=255)                                        gttgttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGcc                                                                               <  2:608207/138‑1 (MQ=255)                                          tgttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTgg                                                                            >  2:735305/1‑139 (MQ=255)                                            ttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGtt                                                                          >  2:573660/1‑139 (MQ=255)                                            ttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGtt                                                                          <  1:735305/139‑1 (MQ=255)                                              aaaCACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTc                                                                        >  1:86092/1‑139 (MQ=255)                                              aaaCACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTc                                                                        >  2:661636/1‑139 (MQ=255)                                                       aCCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑c                                                              <  2:82218/139‑1 (MQ=255)                                                           gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAgg                                                           >  2:633142/1‑138 (MQ=255)                                                                  caACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCtt                                                   <  1:573660/139‑1 (MQ=255)                                                                   aaCCATTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTa                                                  <  2:73828/139‑1 (MQ=255)                                                                          gACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGcc                                                                                           >  2:23551/1‑92 (MQ=255)                                                                          gACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGcc                                                                                           <  1:23551/92‑1 (MQ=255)                                                                                 cAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAAccgc                                    >  1:550184/1‑139 (MQ=255)                                                                                   aCCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAg                                                                                                 <  2:598762/77‑1 (MQ=255)                                                                                   aCCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAg                                                                                                 >  1:598762/1‑77 (MQ=255)                                                                                       gCGTGTAGATAAACAGGGTATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTCTTGCTTAAACATCATAACCGCCGctct                              >  2:282259/1‑139 (MQ=255)                                                                                       gCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGctct                              >  1:808646/1‑139 (MQ=255)                                                                                            aaaaaaaaCGGGGAAAAATCATGGCTTCCCTTTG‑GGCCCTTTAGAACCCCCCAAACCCCCGAGTTGAGTTTCCAAAAAATGATCCCTGTTTTCTTTCCTTG‑CGGGTTTTGTTAAAACATCAAAACCGCCGCTCTTAGt                          <  2:332539/133‑1 (MQ=255)                                                                                              gATAAACGGGGTATAATCAATGCTCCCCTTTG‑AGGCCAGTAGGAACCCCCCAATCCCCGAGATCAGGTTGCAAAAGATGATGCCTGTTTTCTTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGca                       <  1:633142/139‑1 (MQ=255)                                                                                              cagaaaCAGGGAATAATAAATGCTGCCTTTGG‑AGGCCAGTAGGAACCCCCCAATCACCGAGATCAGGTTGCAAAAGATGATGCTTGTTTTCTTTACTTG‑CAGGTTTTGCTTAAACATCAAAACCGCCGCTCTTAGTGca                       <  1:87399/136‑1 (MQ=255)                                                                                                 aaaCGGGGGAAAATAAGTGCT‑CCCTTTGTAGGCCATTAGAATCCCCCCAAGCCCGGAGATCA‑GTTGCCAAAGACGCTTCCCGGTTTTGTTTTTTGTCGGGTTTTTCTAAAACTGCATAACCGCCGCTCTTAGTGcatca                    <  1:78745/139‑1 (MQ=255)                                                                                                 aaaCGGGGAAAAAACAATGCTGCCCTTTG‑AGGCAAGTAGGAACCCCCCAACCCCCGAGATCAGGTTGCAAAAGATGATGCCTGGTTCCTTTACTTG‑CAGTTATTGTTAAAACATCATAACCGCCGCTCTTAGTGcatca                    <  1:663375/139‑1 (MQ=255)                                                                                                   aCAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGcatcat                   >  1:301044/1‑138 (MQ=255)                                                                                                      ggggATAATCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtg               >  2:6941/1‑139 (MQ=255)                                                                                                            aaTCAATGCTGCCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtgttgctg         >  2:87399/1‑136 (MQ=255)                                                                                                                  tgctgcCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAgg   >  1:809912/1‑139 (MQ=255)                                                                                                                   gctgcCCTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGt  >  1:5748/1‑139 (MQ=255)                                                                                                                       cccTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTa                                                         >  1:16060/1‑80 (MQ=255)                                                                                                                       cccTTTG‑AGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTa                                                         <  2:16060/80‑1 (MQ=255)                                                                                                                                      |                                                                                                                          CGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTTG‑AGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTG‑CAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGT  >  NZ_CP009273/442949‑443201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑