Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	449,442	A→G	V61A (GTC→GCC)	yajG ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	449,442	0	A	G	100.0%	145.3 / NA	39	V61A (GTC→GCC)	yajG	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

ATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTG  >  NZ_CP009273/449307‑449577                                                                                                                                        |                                                                                                                                        atgatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCtt                                                                                                                                      >  1:526671/1‑139 (MQ=255)       tGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGcc                                                                                                                                 <  2:93442/138‑1 (MQ=255)             ttAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCt                                                                                                                          <  2:620722/139‑1 (MQ=255)                aCCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGAt                                                                                                                       <  2:787205/139‑1 (MQ=255)                aCCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGAt                                                                                                                       <  2:823978/139‑1 (MQ=255)                       cATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACg                                                                                                                <  1:351405/139‑1 (MQ=255)                          tCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGCTTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCt                                                                                                              >  2:683282/1‑138 (MQ=255)                                caaccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGc                                                                                                       <  2:526671/139‑1 (MQ=255)                                   ccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCAc                                                                                                     >  1:595642/1‑138 (MQ=255)                                            cACGCGCGGTCATCTGTTTTTCCAGCCCTTCTTGCAGCAGGAAACGCCGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCt                                                                                           <  1:395268/139‑1 (MQ=255)                                             aCGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCt                                                                                           >  2:364051/1‑138 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTg                                                                                                             >  1:301571/1‑119 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTg                                                                                                             <  2:301571/119‑1 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTa                                                                                         >  1:291351/1‑139 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTa                                                                                         >  1:437885/1‑139 (MQ=255)                                                    gTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGa                                                                                   <  2:765115/139‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:80854/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:753318/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:486838/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:621605/138‑1 (MQ=255)                                                                 ccAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCt                                                                      >  1:295826/1‑139 (MQ=255)                                                                    gCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTg                                                                    >  2:543723/1‑138 (MQ=255)                                                                      aCTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGac                                                                 <  1:543723/139‑2 (MQ=255)                                                                      aCTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGac                                                                 <  1:364051/139‑2 (MQ=255)                                                                      aCTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGAt                                                                 <  2:437885/139‑1 (MQ=255)                                                                                    gAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                                   >  2:744491/1‑139 (MQ=255)                                                                                        cGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTaa                                               <  1:683282/139‑1 (MQ=255)                                                                                            gATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcgt                                           <  2:362085/139‑1 (MQ=255)                                                                                              tCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGACCCTGCTGTGGCAGTGTAAtcgtcg                                         >  1:662493/1‑139 (MQ=255)                                                                                                          gTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGACCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCaa                             <  2:662493/139‑1 (MQ=255)                                                                                                                    cGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTgg                   >  1:625381/1‑139 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAgg                                                                        >  1:174850/1‑81 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAgg                                                                        <  2:174850/81‑1 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGc                                                                       >  2:96652/1‑82 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGc                                                                       <  1:96652/82‑1 (MQ=255)                                                                                                                               tCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTgcg         <  1:461901/138‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:154567/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  1:48837/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGACCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:295826/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTG  >  NZ_CP009273/449307‑449577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑