Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	547,771	T→C	T94A (ACT→GCT)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	547,771	0	T	C	100.0%	175.6 / NA	47	T94A (ACT→GCT)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

TTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATC  >  NZ_CP009273/547636‑547904                                                                                                                                        |                                                                                                                                      tttCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCgg                                                                                                                                    <  1:105681/139‑1 (MQ=255) tttCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCgg                                                                                                                                    <  1:669491/139‑1 (MQ=255)   tCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGc                                                                                                                                   <  1:480826/138‑1 (MQ=255)        tGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              <  2:126370/138‑1 (MQ=255)                       ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              <  2:786379/123‑1 (MQ=255)                       ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTg                                                                                                                              >  1:786379/1‑123 (MQ=255)                       ttGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              >  1:692224/1‑139 (MQ=255)                         gACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgct                                                                                                             <  2:482141/138‑1 (MQ=255)                               gACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCt                                                                                                       >  2:649177/1‑138 (MQ=255)                                             ccaGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  2:283926/1‑139 (MQ=255)                                              caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  2:854408/1‑138 (MQ=255)                                              caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                        >  1:762780/1‑138 (MQ=255)                                               aGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCa                                                                                      <  1:649177/139‑1 (MQ=255)                                                gTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaa                                                                                     <  2:692224/139‑1 (MQ=255)                                                   cGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataa                                                                                  >  2:659859/1‑139 (MQ=255)                                                                  gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgc                                                                   >  1:548361/1‑139 (MQ=255)                                                                  gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgc                                                                   >  1:514587/1‑139 (MQ=255)                                                                  gCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    <  2:686038/138‑1 (MQ=255)                                                                   ccAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcgcg                                                                  >  1:695079/1‑139 (MQ=255)                                                                       cTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGtt                                                               >  1:203813/1‑138 (MQ=255)                                                                                 cGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataat                                                                                 >  2:356037/1‑110 (MQ=255)                                                                                 cGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaataat                                                                                 <  1:356037/110‑1 (MQ=255)                                                                                    tCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGAt                                                 >  2:55622/1‑139 (MQ=255)                                                                                    tCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGAt                                                 >  1:115910/1‑139 (MQ=255)                                                                                      aaaCACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcac                                                            <  2:334249/126‑1 (MQ=255)                                                                                      aaaCACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcac                                                            >  1:334249/1‑126 (MQ=255)                                                                                             gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    >  2:461005/1‑111 (MQ=255)                                                                                             gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATcg                                                                    <  1:461005/111‑1 (MQ=255)                                                                                              cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  1:836797/1‑139 (MQ=255)                                                                                              cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  2:453757/1‑139 (MQ=255)                                                                                              cAGGCCACTCGCTGCGATCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                       >  1:822109/1‑139 (MQ=255)                                                                                                 gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTc                                    <  1:659859/139‑1 (MQ=255)                                                                                                  ccACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                   <  1:283926/139‑1 (MQ=255)                                                                                                      tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                               >  1:589947/1‑139 (MQ=255)                                                                                                      tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                               >  2:680282/1‑139 (MQ=255)                                                                                                             gTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGc                        <  2:548361/139‑1 (MQ=255)                                                                                                              ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCg                       >  2:831112/1‑139 (MQ=255)                                                                                                                   cAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              >  1:804453/1‑47 (MQ=255)                                                                                                                   cAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgc                                                                                                              <  2:804453/47‑1 (MQ=255)                                                                                                                       tAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc              <  2:822109/139‑1 (MQ=255)                                                                                                                       tAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc              <  2:848523/139‑1 (MQ=255)                                                                                                                         aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGa                                                                                               <  1:2979/56‑1 (MQ=255)                                                                                                                         aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGa                                                                                               >  2:2979/1‑56 (MQ=255)                                                                                                                         aCTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGcc             <  2:695079/138‑1 (MQ=255)                                                                                                                          cTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAg           <  1:55622/139‑1 (MQ=255)                                                                                                                               aCGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTc      <  2:589947/139‑1 (MQ=255)                                                                                                                                   tGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtc  >  1:623764/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      TTTCATTTGCGCGTAAACGCCATTGACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATC  >  NZ_CP009273/547636‑547904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑