Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	633,831	C→T	A67T (GCT→ACT)	dsbG ←	thiol:disulfide interchange protein DsbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	633,831	0	C	T	100.0%	90.0 / NA	24	A67T (GCT→ACT)	dsbG	thiol:disulfide interchange protein DsbG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/17);  total (7/17)

GAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTG  >  NZ_CP009273/633706‑633962                                                                                                                              |                                                                                                                                    gaggagCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATc                                                                                                                         <  2:336809/138‑1 (MQ=255)      gCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGt                                                                                                                   <  2:571085/139‑1 (MQ=255)         agggggATTGTTCCATCCGTTGCCACATTTCGCGTCCTGAGGGGGCGTCACATTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAg                                                                                                                >  1:282344/4‑139 (MQ=255)         aGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAg                                                                                                                >  2:326077/1‑139 (MQ=255)                   ttCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTg                                                                                                      <  1:171222/139‑1 (MQ=255)                     ccATCCGTTGCCACCTTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGCGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa                                                                                                     <  2:608037/138‑1 (MQ=255)                     ccATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa                                                                                                     <  1:326077/138‑1 (MQ=255)                     ccATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa                                                                                                     <  2:677141/138‑1 (MQ=255)                          cGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCa                                                                                               <  2:547165/139‑1 (MQ=255)                             tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCatat                                                                                            >  2:685492/1‑139 (MQ=255)                                               gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGt                                                                                                                   <  2:854507/98‑1 (MQ=255)                                               gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGt                                                                                                                   >  1:854507/1‑98 (MQ=255)                                               gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGAt                                                                          <  2:348276/139‑1 (MQ=255)                                               gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGAt                                                                          <  1:562297/139‑1 (MQ=255)                                                cTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATTTTGTGTCTGGTAGATGGTGATGCTCATATCCTGATACTTTCCGAGATa                                                                         <  2:282344/139‑1 (MQ=255)                                                   gTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAAcc                                                                      <  1:259434/139‑1 (MQ=255)                                                                          aGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGa                                               <  2:15134/139‑1 (MQ=255)                                                                          aGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGa                                               <  1:685492/139‑1 (MQ=255)                                                                                ttACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAg                                                                                                            >  2:352408/1‑72 (MQ=255)                                                                                ttACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAg                                                                                                            <  1:352408/72‑1 (MQ=255)                                                                                      aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTtgatga                                   >  2:372350/1‑139 (MQ=255)                                                                                                           tACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCaa              <  2:754080/139‑1 (MQ=255)                                                                                                           tACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCa               <  1:66272/138‑1 (MQ=255)                                                                                                                       gagaTAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTg  >  1:658387/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                    GAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACTG  >  NZ_CP009273/633706‑633962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑