Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	640,333	A→C	W43G (TGG→GGG)	rna ←	ribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	640,333	0	A	C	100.0%	173.5 / NA	47	W43G (TGG→GGG)	rna	ribonuclease I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/24);  total (23/24)

CGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCAGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGG  >  NZ_CP009273/640199‑640464                                                                                                                                       |                                                                                                                                    cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  2:38925/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  1:26847/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  1:653217/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  2:689615/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  2:45417/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  2:517073/139‑1 (MQ=255) cGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  2:549605/139‑1 (MQ=255)   aTTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGagag                                                                                                                               >  1:554958/1‑139 (MQ=255)       aGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGc                                                                                                                            <  2:23519/138‑1 (MQ=255)           aaTCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGa                                                                                                                       <  1:112268/139‑1 (MQ=255)            aTCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTCACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGa                                                                                                                       >  2:20739/1‑138 (MQ=255)                  gCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACTTAGc                                                                                                                 >  1:436095/1‑138 (MQ=255)                       aGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCa                                                                                                            >  2:463277/1‑138 (MQ=255)                         aCCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCaaaa                                                                                                         <  1:31196/139‑1 (MQ=255)                         aCCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCaaaa                                                                                                         >  1:247115/1‑139 (MQ=255)                             tGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGc                                                                                                                            <  1:447509/116‑1 (MQ=255)                             tGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGc                                                                                                                            >  2:447509/1‑116 (MQ=255)                             tGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGc                                                                                                     >  1:363810/1‑139 (MQ=255)                                   gTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  1:409434/105‑1 (MQ=255)                                   gTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 >  2:409434/1‑105 (MQ=255)                                   gTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACt                                                                                               >  2:843550/1‑139 (MQ=255)                                    tCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTg                                                                                              >  2:453758/1‑139 (MQ=255)                                       gAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 <  1:422660/101‑1 (MQ=255)                                       gAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGgag                                                                                                                                 >  2:422660/1‑101 (MQ=255)                                        aaaaTCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGAC‑TAGCGATCAAAATCGCCATACTGTTTTg                                                                                         >  1:127351/1‑139 (MQ=255)                                              aGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGc                                                                                    <  2:510977/139‑1 (MQ=255)                                                          cGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCg                                                                        >  2:585725/1‑139 (MQ=255)                                                                 gTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCag                                                                 <  2:247115/139‑1 (MQ=255)                                                                    tGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAggg                                                                                                                             <  2:307172/76‑1 (MQ=255)                                                                    tGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAggg                                                                                                                             >  1:307172/1‑76 (MQ=255)                                                                                       gttcgttACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCt                                                                              <  2:355444/104‑1 (MQ=255)                                                                                       gttcgttACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCt                                                                              >  1:355444/1‑104 (MQ=255)                                                                                       gttcgttACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCagag                                               <  2:725038/135‑1 (MQ=255)                                                                                       gttcgttACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAAc                                           >  2:96387/1‑139 (MQ=255)                                                                                        ttcgttACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAAcc                                          >  2:754991/1‑139 (MQ=255)                                                                                                     cGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACgcgg                             >  1:753471/1‑139 (MQ=255)                                                                                                                    tGGCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGaa                <  1:463277/137‑1 (MQ=255)                                                                                                                     ggCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGc             >  1:420748/1‑139 (MQ=255)                                                                                                                     ggCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGc             >  1:800942/1‑139 (MQ=255)                                                                                                                      gCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAAt               <  2:440645/136‑1 (MQ=255)                                                                                                                      gCAAAATCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCt            <  2:800942/139‑1 (MQ=255)                                                                                                                            tCCGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCAt       >  1:451087/1‑138 (MQ=255)                                                                                                                             ccGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATaa     <  2:362503/139‑1 (MQ=255)                                                                                                                              cGGTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAAt    >  1:515837/1‑139 (MQ=255)                                                                                                                              cGGTTTGCCCGGAGAGGGCCAGGAC‑TAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAAt    <  2:127351/138‑1 (MQ=255)                                                                                                                              cGGTCTGCTCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTCTCATAAt    <  2:783724/139‑1 (MQ=255)                                                                                                                                gTTTGCCCGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATgg  >  1:505820/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    CGATTTAGGCAATCCTGGCCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTTGCAGGCGACATTCATCTCGTTCGTTACGATTTCGATCGTGTTGACTCTGGCAAAATCCGGTTTGCCAGGAGAGGGCCAGGACATAGCGATCAAAATCGCCATACTGTTTTGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGG  >  NZ_CP009273/640199‑640464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑