| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 753,637 | A→G | N165D (AAT→GAT) | sdhB → | succinate dehydrogenase iron‑sulfur subunit SdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 753,637 | 0 | A | G | 97.7% | 149.6 / ‑6.4 | 44 | N165D (AAT→GAT) | sdhB | succinate dehydrogenase iron‑sulfur subunit SdhB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/18); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACA > NZ_CP009273/753509‑753766 | tGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 1:690730/1‑137 (MQ=255) gAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAg > 1:695234/1‑139 (MQ=255) aaaaTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGccc > 2:621341/1‑139 (MQ=255) tttACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat < 2:839079/139‑1 (MQ=255) gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 2:404966/1‑94 (MQ=255) gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt < 1:404966/94‑1 (MQ=255) gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATTTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 1:644065/139‑1 (MQ=255) gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 1:281006/139‑1 (MQ=255) gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat < 2:796527/139‑1 (MQ=255) agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 2:103191/139‑1 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTTCTGATTGATAGcc > 1:680512/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCGTGATTGATTGcc > 1:662775/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTATAGcc > 1:328484/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:229423/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:116559/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 2:662002/1‑139 (MQ=255) tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAgc < 1:662002/138‑1 (MQ=255) aTGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTc < 2:229423/139‑1 (MQ=255) tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCg < 2:116559/139‑1 (MQ=255) tATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc > 1:256888/1‑139 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAg > 2:671905/1‑106 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 1:671905/106‑1 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa < 1:122775/126‑1 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACa > 2:122775/1‑126 (MQ=255) ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGTTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGt < 1:191986/138‑1 (MQ=255) ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGtt > 2:126046/1‑139 (MQ=255) ctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTg > 1:219755/1‑139 (MQ=255) ctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGGGATACCGAGACTGACAGCCGCCTCGACGGTTTg > 2:613832/1‑139 (MQ=255) tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt < 1:76931/48‑1 (MQ=255) tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 2:76931/1‑48 (MQ=255) tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTATCGGCATATCGTTTTCTGATTTATAGCCGTGATTCCGAGTATGACAGCCGCCTCGACGGgtt > 2:474489/1‑137 (MQ=255) gcgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCCGGCTTGTTAGCGGCATATCGTTTCCTTATTTATAGCCGTGATACCGAGACTTACAGCCGCCTCGACGGTTTGAg > 2:86924/1‑139 (MQ=255) cccATGTTGTTCAACCTCTTGTCCATCTTTCTGGTGGAATCCCGATAAGTTTAGCGGCCCGGCAGGCTTGTTAGCCGCATGTCGTTTCCGGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGa < 1:193477/135‑1 (MQ=255) ccTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGGGACTGACAGCCGCCCCGACGGGTTGAGTGATGCATTCAGCg > 2:437953/1‑138 (MQ=255) ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 1:790508/82‑1 (MQ=255) ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg > 2:790508/1‑82 (MQ=255) ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTAtt > 1:138634/1‑139 (MQ=255) cGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTg > 1:736058/1‑139 (MQ=255) tCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc > 2:70225/1‑105 (MQ=255) tCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc < 1:70225/105‑1 (MQ=255) cTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACGGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTca < 2:476637/139‑1 (MQ=255) ttCTGGTGGGATCCCGATAAGTTTATCGGCCCGGGAGGCCTGTTTGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTTAGCGGATTTCGCTGTca > 2:8402/1‑137 (MQ=255) tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 2:256888/138‑1 (MQ=255) tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 1:616701/138‑1 (MQ=255) | TGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACA > NZ_CP009273/753509‑753766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |