Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	798,635	T→C	G431G (GGT→GGC)	ybhI →	anion permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	798,635	0	T	C	100.0%	153.8 / NA	45	G431G (GGT→GGC)	ybhI	anion permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

CTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAA  >  NZ_CP009273/798500‑798766                                                                                                                                        |                                                                                                                                    cTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCg                                                                                                                                  <  2:277842/139‑1 (MQ=255)    tatCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCCGt                                                                                                                                <  1:629608/138‑1 (MQ=255)         ttGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCtt                                                                                                                          >  1:512481/1‑139 (MQ=255)          tGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGCCATCttt                                                                                                                         >  2:575381/1‑139 (MQ=255)            cTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:43396/130‑1 (MQ=255)            cTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:43396/1‑130 (MQ=255)                   aCCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCAGCTTTGGCGTGGGt                                                                                                                >  2:182622/1‑139 (MQ=255)                          tttGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACg                                                                                                         <  2:12274/139‑1 (MQ=255)                             gCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGata                                                                                                      >  2:848713/1‑139 (MQ=255)                                                 cGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGtggttggt                                                                                   <  2:512481/138‑1 (MQ=255)                                                     gcACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:848713/139‑1 (MQ=255)                                                     gcACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:150952/139‑1 (MQ=255)                                                           ttAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     <  1:570138/94‑1 (MQ=255)                                                           ttAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     >  2:570138/1‑94 (MQ=255)                                                              aTGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTg                                                                      <  1:72127/138‑1 (MQ=255)                                                              aTGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     <  1:610342/139‑1 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCCTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCCTCTTTGGGGGGGGGTATTACGATATTAAATCCTGGTGGTTGGTCGGTGCGGGACTGCAGata                                                                >  2:72127/1‑139 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:5389/1‑75 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:5389/75‑1 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                >  1:57208/1‑75 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGt                                                                                                                                <  2:57208/75‑1 (MQ=255)                                                                   aaCCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGata                                                                >  2:126492/1‑139 (MQ=255)                                                                        cgcTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAAc                                                           >  2:436128/1‑139 (MQ=255)                                                                               aCTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTg                                                    <  2:443684/139‑1 (MQ=255)                                                                                  gttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     <  2:561295/71‑1 (MQ=255)                                                                                  gttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCg                                                                                                                     >  1:561295/1‑71 (MQ=255)                                                                                   ttgttCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGGGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTg                                                 >  1:697099/1‑138 (MQ=255)                                                                                                    tGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  1:148557/139‑1 (MQ=255)                                                                                                    tGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  1:558354/139‑1 (MQ=255)                                                                                                           aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCt                                      >  1:135665/1‑125 (MQ=255)                                                                                                           aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCt                                      <  2:135665/125‑1 (MQ=255)                                                                                                           aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               <  2:300632/132‑1 (MQ=255)                                                                                                           aTGGTTACTCACTATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCgtg                               >  1:300632/1‑132 (MQ=255)                                                                                                                       tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCAccc                                       >  2:212956/1‑112 (MQ=255)                                                                                                                       tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCAccc                                       <  1:212956/112‑1 (MQ=255)                                                                                                                       tATGGCGGCGCGGCAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGa            >  1:208742/1‑139 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              >  2:438609/1‑65 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTg                                                                              <  1:438609/65‑1 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTcc                                                      <  1:196372/89‑1 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTcc                                                      >  2:196372/1‑89 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTg     >  2:140266/1‑138 (MQ=255)                                                                                                                               cgcggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTg     >  1:28528/1‑138 (MQ=255)                                                                                                                                 cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     >  1:715261/1‑72 (MQ=255)                                                                                                                                 cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGa                                                                     <  2:715261/72‑1 (MQ=255)                                                                                                                                 cggcAGGCCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGGGGTGGAATATGCTGATCGGCTGGaa  >  1:447655/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    CTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAA  >  NZ_CP009273/798500‑798766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑