Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	918,237	T→A	N14K (AAT→AAA)	yljB →	protein YljB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	918,237	0	T	A	100.0%	143.1 / NA	37	N14K (AAT→AAA)	yljB	protein YljB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/17);  total (20/17)

GGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAAT  >  NZ_CP009273/918106‑918370                                                                                                                                    |                                                                                                                                      ggggCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGa                                                                                                                                   >  2:681468/1‑136 (MQ=255) ggggCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGa                                                                                                                                   >  2:617598/1‑136 (MQ=255)        ttCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGc                                                                                                                         >  1:532594/1‑139 (MQ=255)         tCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAagtg                                                                                                                                    >  2:50859/1‑127 (MQ=255)         tCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAagtg                                                                                                                                    <  1:50859/127‑1 (MQ=255)                  cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAAc                                                                                                               >  2:92814/1‑139 (MQ=255)                  cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAAc                                                                                                               >  1:840704/1‑139 (MQ=255)                  cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAAc                                                                                                               >  1:478747/1‑139 (MQ=255)                  cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAAc                                                                                                               >  1:378567/1‑139 (MQ=255)                  cGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGAGCAGATATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAAc                                                                                                               >  2:586166/1‑139 (MQ=255)                             gAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAgatga                                                                                                      >  1:581301/1‑137 (MQ=255)                                aTACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGtt                                                                                                 <  1:488846/139‑1 (MQ=255)                                aTACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGtt                                                                                                 <  1:681468/139‑1 (MQ=255)                                     aTTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtatcta                                                                                            <  1:27791/139‑1 (MQ=255)                                     aTTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTtatcta                                                                                            <  2:532594/139‑1 (MQ=255)                                          gagaATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCAt                                                                                        <  1:586166/138‑1 (MQ=255)                                              aTTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGa                                                                                   <  1:92814/139‑1 (MQ=255)                                                     cGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGAtt                                                                            >  1:636630/1‑139 (MQ=255)                                                       aGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCaa                                                                                                                >  2:655225/1‑101 (MQ=255)                                                       aGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCaa                                                                                                                <  1:655225/101‑1 (MQ=255)                                                            tCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAgg                                                                     <  2:770968/139‑1 (MQ=255)                                                            tCAAGCATTTGACAGGGGACAAGAGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAgg                                                                     <  1:253722/139‑1 (MQ=255)                                                                     tGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAg                                                             <  1:123475/138‑1 (MQ=255)                                                                      gACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGct                                                           >  2:420132/1‑139 (MQ=255)                                                                      gACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGct                                                           >  1:211310/1‑139 (MQ=255)                                                                           gggACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGt                                                      >  2:640702/1‑139 (MQ=255)                                                                           gggACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGt                                                      >  2:720423/1‑139 (MQ=255)                                                                            ggACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGt                                                      >  1:233883/1‑138 (MQ=255)                                                                                     gCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGa                                                                                   <  2:589395/100‑1 (MQ=255)                                                                                     gCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGa                                                                                   >  1:589395/1‑100 (MQ=255)                                                                                      cAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGa                                                                                                             >  2:116898/1‑73 (MQ=255)                                                                                      cAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGa                                                                                                             <  1:116898/73‑1 (MQ=255)                                                                                          tATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGtt                                       <  1:720423/139‑1 (MQ=255)                                                                                          tATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGtt                                       <  1:617598/139‑1 (MQ=255)                                                                                          tATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGtt                                       <  2:840704/139‑1 (MQ=255)                                                                                              aaTCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAg                                                                      >  2:532921/1‑104 (MQ=255)                                                                                              aaTCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAg                                                                      <  1:532921/104‑1 (MQ=255)                                                                                                                          ttGACAGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAAc         <  2:233883/137‑1 (MQ=255)                                                                                                                               aGGAAAGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAAt  >  2:207435/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      GGGGCTGTTCAAAACCTCGCCAACTCTAGAAATACAATTTAGAGAATTAGGGCGAGCCGTCAAGCATTTGACAGGGGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAAT  >  NZ_CP009273/918106‑918370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑