Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,006,907	T→C	D496D (GAT→GAC)	uup →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,006,907	0	T	C	100.0%	70.7 / NA	21	D496D (GAT→GAC)	uup	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

CTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGATCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCT  >  NZ_CP009273/1006785‑1007036                                                                                                                           |                                                                                                                                  cTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGAt                                                                                                                    <  1:477799/138‑1 (MQ=255) cTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGAt                                                                                                                    <  2:726361/138‑1 (MQ=255)    aaaCCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATaa                                                                                                                  <  2:778390/137‑1 (MQ=255)    aaaCCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAaca                                                                                                                <  2:345771/139‑1 (MQ=255)           gCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGAt                                                                                                                    >  1:337278/1‑128 (MQ=255)           gCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGAt                                                                                                                    <  2:337278/128‑1 (MQ=255)            cAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACa                                                                                                        <  1:823175/139‑1 (MQ=255)                         cTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCt                                                                                           >  2:502060/1‑139 (MQ=255)                         cTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCt                                                                                           >  1:166673/1‑139 (MQ=255)                                 aCCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTAc                                                                                                         >  1:359911/1‑117 (MQ=255)                                 aCCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTAc                                                                                                         <  2:359911/117‑1 (MQ=255)                                                           cGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTcgg                                                         <  2:253376/139‑1 (MQ=255)                                                                      ctgGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCatg                                              >  2:492042/1‑139 (MQ=255)                                                                          aagaaCTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGc                                          <  2:166673/139‑1 (MQ=255)                                                                                    cGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAAtt                                                                       <  1:803207/100‑1 (MQ=255)                                                                                    cGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAAtt                                                                       >  2:803207/1‑100 (MQ=255)                                                                                                 ggCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTAtg                   >  1:689643/1‑139 (MQ=255)                                                                                                          ttGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTc           <  1:345298/138‑1 (MQ=255)                                                                                                                  tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTc                                                           <  1:839434/82‑1 (MQ=255)                                                                                                                  tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTc                                                           >  2:839434/1‑82 (MQ=255)                                                                                                                  tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCACCAAGAGAAGCATGTGGGGCTCAAACAAccc  >  1:74063/1‑138 (MQ=255)                                                                                                                           |                                                                                                                                  CTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGATCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCT  >  NZ_CP009273/1006785‑1007036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑