Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,017,704	C→A	A549S (GCC→TCC)	yccS ←	YccS family putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,017,704	0	C	A	100.0%	143.8 / NA	37	A549S (GCC→TCC)	yccS	YccS family putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/17);  total (20/17)

CGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAG  >  NZ_CP009273/1017598‑1017836                                                                                                           |                                                                                                                                     cGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACt                                                                                                      >  1:739453/1‑139 (MQ=255)       tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  1:815767/139‑1 (MQ=255)       tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                >  1:521206/1‑139 (MQ=255)       tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  2:547276/139‑1 (MQ=255)         tgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                >  1:764140/1‑137 (MQ=255)            aTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGc                                                                                           >  2:511119/1‑139 (MQ=255)             tCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          <  2:268941/139‑1 (MQ=255)                   cggGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     >  2:412370/1‑138 (MQ=255)                                      gACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTg                                                                                                     <  2:751207/103‑1 (MQ=255)                                      gACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTg                                                                                                     >  1:751207/1‑103 (MQ=255)                                       aCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCa                                                                <  1:250180/139‑1 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                            >  2:395300/1‑97 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                            <  1:644674/97‑1 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                            >  2:644674/1‑97 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAgg                                                                                            <  1:395300/97‑1 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGca                                                          <  1:355299/131‑1 (MQ=255)                                                     ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGca                                                          >  2:355299/1‑131 (MQ=255)                                                                tGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          <  1:465301/88‑1 (MQ=255)                                                                tGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          >  2:465301/1‑88 (MQ=255)                                                                     cAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  <  2:521206/139‑1 (MQ=255)                                                                            gCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgcc                                                                      >  1:749256/1‑96 (MQ=255)                                                                            gCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgcc                                                                      <  2:749256/96‑1 (MQ=255)                                                                            gCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGt                            >  2:842961/1‑138 (MQ=255)                                                                             cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGa                                       <  2:528023/126‑1 (MQ=255)                                                                             cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGa                                       >  1:528023/1‑126 (MQ=255)                                                                                 gagaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt                      <  2:764140/139‑1 (MQ=255)                                                                                  agaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     >  2:230011/1‑75 (MQ=255)                                                                                  agaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     <  1:230011/75‑1 (MQ=255)                                                                                      aCCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          <  2:474000/66‑1 (MQ=255)                                                                                      aCCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          >  1:474000/1‑66 (MQ=255)                                                                                       ccGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCa                <  1:412370/139‑1 (MQ=255)                                                                                          aCAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  >  1:709240/1‑118 (MQ=255)                                                                                          aCAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  <  2:709240/118‑1 (MQ=255)                                                                                             gTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCt          >  1:480189/1‑139 (MQ=255)                                                                                              ttGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCtt         >  1:642654/1‑139 (MQ=255)                                                                                                      tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAg  >  2:175900/1‑138 (MQ=255)                                                                                                      tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAg  >  2:4736/1‑138 (MQ=255)                                                                                                           |                                                                                                                                     CGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAG  >  NZ_CP009273/1017598‑1017836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑