Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,069,819	T→G	L41R (CTG→CGG)	rutR →	HTH‑type transcriptional regulator RutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,069,819	0	T	G	100.0%	119.2 / NA	33	L41R (CTG→CGG)	rutR	HTH‑type transcriptional regulator RutR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/18);  total (15/18)

GGGGAGAGCGCATGACGCAAGGCGCAGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATAT  >  NZ_CP009273/1069687‑1069924                                                                                                                                     |                                                                                                          ggggAGAGCGCATGACGCAAGGCGCAGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCa                                                                                                     <  1:504648/139‑1 (MQ=255)  gggAGAGCGCATGACGCAAGGCGCAGTGAAAACAACGGGCAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAg                                                                                                       >  2:810102/1‑136 (MQ=255)                          aGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGc                                                                                                      <  1:124079/113‑1 (MQ=255)                          aGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGc                                                                                                      >  2:124079/1‑113 (MQ=255)                          aGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTc                                                                             >  2:756739/1‑138 (MQ=255)                          aGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTc                                                                             >  2:101217/1‑138 (MQ=255)                                    acGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAATCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATc                                                                  <  2:675847/139‑1 (MQ=255)                                      gggTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATctg                                                                >  2:98169/1‑139 (MQ=255)                                                 cgcgcgCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCa                                                                                                     <  1:640617/91‑1 (MQ=255)                                                 cgcgcgCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCa                                                                                                     >  2:640617/1‑91 (MQ=255)                                                              cgcgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAagag                                        <  1:626622/139‑1 (MQ=255)                                                               gcgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAgg                                       <  1:810102/139‑1 (MQ=255)                                                                cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgc                                      >  1:226234/1‑139 (MQ=255)                                                                cgAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgc                                      >  1:68192/1‑139 (MQ=255)                                                                 gaagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  2:476331/139‑1 (MQ=255)                                                                 gaagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  1:541673/139‑1 (MQ=255)                                                                 gaagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  1:98169/139‑1 (MQ=255)                                                                  aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcgc                                    >  2:90012/1‑139 (MQ=255)                                                                  aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcgc                                    <  2:68192/139‑1 (MQ=255)                                                                  aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  2:521622/138‑1 (MQ=255)                                                                  aagaaAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     >  1:521622/1‑138 (MQ=255)                                                                                                gACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     >  1:672114/1‑108 (MQ=255)                                                                                                gACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  2:672114/108‑1 (MQ=255)                                                                                                    cTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGatat  <  1:90012/139‑1 (MQ=255)                                                                                                    cTTTTTCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGatat  <  2:486596/139‑1 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAagag                                        <  1:714682/100‑1 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAagag                                        >  2:714682/1‑100 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     >  1:413973/1‑103 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  2:413973/103‑1 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     >  2:172466/1‑103 (MQ=255)                                                                                                     tttttCACAATTCGGTTTTCACGGCACAAGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGgcg                                     <  1:172466/103‑1 (MQ=255)                                                                                                                                aaGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAagag                                        <  1:60985/73‑1 (MQ=255)                                                                                                                                aaGGCGGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAagag                                        >  2:60985/1‑73 (MQ=255)                                                                                                                                     |                                                                                                          GGGGAGAGCGCATGACGCAAGGCGCAGTGAAAACAACGGGTAAACGTTCGCGCGCAGTAAGCGCGAAGAAAAAAGCGATTCTTAGCGCAGCACTGGACACTTTTTCACAATTCGGTTTTCACGGCACAAGGCTGGAGCAGATCGCAGAGTTGGCGGGTGTTTCAAAAACCAATCTGCTGTATTACTTTCCGTCAAAAGAGGCGCTGTATATTGCCGTGCTGCGGCAGATTCTCGATAT  >  NZ_CP009273/1069687‑1069924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑