Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,121,190	G→A	G58D (GGC→GAC)	rimJ →	ribosomal protein S5‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,121,190	0	G	A	100.0%	143.5 / NA	37	G58D (GGC→GAC)	rimJ	ribosomal protein S5‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

AACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCA  >  NZ_CP009273/1121056‑1121325                                                                                                                                       |                                                                                                                                        aaCCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTg                                                                                                                                      <  1:657313/138‑1 (MQ=255)      caGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcaggc                                                                                                                                <  1:309863/139‑1 (MQ=255)      caGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcaggc                                                                                                                                <  2:311759/139‑1 (MQ=255)           cGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcaggccaggc                                                                                                                           >  2:229188/1‑139 (MQ=255)                     tGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcaggcc                                                                                                                               >  1:25953/1‑125 (MQ=255)                     tGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcaggcc                                                                                                                               <  2:25953/125‑1 (MQ=255)                     tGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGgatgat                                                                                                                 >  2:827769/1‑139 (MQ=255)                             gTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGa                                                                                                           <  1:229188/137‑1 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcagg                                                                                                                                 <  1:197684/104‑1 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGcagg                                                                                                                                 >  2:197684/1‑104 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTggg                                                                                                                       <  1:502941/114‑1 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTggg                                                                                                                       >  2:502941/1‑114 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATaaa                                                                                                 >  2:254659/1‑136 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATaaa                                                                                                 <  1:254659/136‑1 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAAc                                                                                                >  2:27630/1‑137 (MQ=255)                                        tGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAAc                                                                                                >  2:268349/1‑137 (MQ=255)                                               tGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAg                                                                                       >  2:642673/1‑139 (MQ=255)                                                   gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCttt                                                                                   <  1:27630/139‑1 (MQ=255)                                                                     cAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGa                                                                 >  2:604310/1‑139 (MQ=255)                                                                        agaATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGAccc                                                              >  2:239940/1‑139 (MQ=255)                                                                                cATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGaaa                                                      <  2:487271/139‑1 (MQ=255)                                                                                  tttCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGaaaaa                                                    <  2:842047/139‑1 (MQ=255)                                                                                        cAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGGTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAagagtt                                               >  2:370538/1‑136 (MQ=255)                                                                                                gggAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGt                                      >  1:801946/1‑139 (MQ=255)                                                                                                    gCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGcc                                  <  1:604310/139‑1 (MQ=255)                                                                                                       aGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGtt                                                                                          >  2:661008/1‑80 (MQ=255)                                                                                                       aGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGtt                                                                                          <  1:661008/80‑1 (MQ=255)                                                                                                       aGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAt                               >  1:87278/1‑139 (MQ=255)                                                                                                       aGTGCGCGACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAt                               >  2:480799/1‑139 (MQ=255)                                                                                                           cgcgACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACt                                                                              >  2:846984/1‑88 (MQ=255)                                                                                                           cgcgACGAAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACt                                                                              <  1:846984/88‑1 (MQ=255)                                                                                                              gacgaAAGCCACTGTTATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCa                        <  2:52555/139‑1 (MQ=255)                                                                                                                            ttATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGt             <  2:142893/136‑1 (MQ=255)                                                                                                                            ttATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGt             >  1:142893/1‑136 (MQ=255)                                                                                                                            ttATCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTgg           <  2:234699/138‑1 (MQ=255)                                                                                                                              aTCCATCAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGct         <  1:268349/138‑1 (MQ=255)                                                                                                                                    cAGACTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGGTTAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCa  >  2:644057/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        AACCACAGACCGACTGGTCGTGCGTCTGGTGCATGATCGTGATGCCTGGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCA  >  NZ_CP009273/1121056‑1121325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑