Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,280,272	G→A	A405T (GCA→ACA)	narH →	nitrate reductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,280,272	0	G	A	100.0%	143.2 / NA	36	A405T (GCA→ACA)	narH	nitrate reductase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/17);  total (19/17)

TCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTG  >  NZ_CP009273/1280137‑1280407                                                                                                                                        |                                                                                                                                        tctcCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGcaca                                                                                                                                       <  2:337801/138‑1 (MQ=255)     cGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGcaca                                                                                                                                       >  2:682528/1‑134 (MQ=255)     cGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGcaca                                                                                                                                       <  1:682528/134‑1 (MQ=255)      gATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGaaa                                                                                                                                 >  1:407281/1‑139 (MQ=255)                       aCGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCg                                                                                                                >  2:798950/1‑139 (MQ=255)                                aGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACa                                                                                                        >  1:49207/1‑138 (MQ=255)                                 gCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACaaa                                                                                                      <  2:130389/139‑1 (MQ=255)                                               gCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACt                                                                                                                                     >  2:116979/1‑94 (MQ=255)                                               gCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACt                                                                                                                                     <  1:116979/94‑1 (MQ=255)                                                       ccAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa                                                                                <  1:54385/139‑1 (MQ=255)                                                                    gCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   <  1:263968/139‑1 (MQ=255)                                                                    gCCTGCGTATTCCGGTACAGTATCGGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   <  1:551408/139‑1 (MQ=255)                                                                         cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTgcgc                                                              >  2:378868/1‑139 (MQ=255)                                                                         cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTgcgc                                                              >  1:513504/1‑139 (MQ=255)                                                                                  gTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAgg                                                     >  1:306670/1‑139 (MQ=255)                                                                                        tATCTGGCGAATCTGCTGACCGCCGGTGATACTAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAggtcggtc                                               >  2:207078/1‑139 (MQ=255)                                                                                        tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAAc                                                                                                     >  1:199876/1‑85 (MQ=255)                                                                                        tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAAc                                                                                                     <  2:199876/85‑1 (MQ=255)                                                                                        tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAggtcggtc                                               >  1:598740/1‑139 (MQ=255)                                                                                        tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAggtcggtc                                               >  1:719172/1‑139 (MQ=255)                                                                                          tCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCt                                              >  1:440938/1‑138 (MQ=255)                                                                                          tCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCt                                              >  1:246545/1‑138 (MQ=255)                                                                                             ggCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa                                                                                <  1:177405/101‑1 (MQ=255)                                                                                             ggCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTa                                                                                >  2:177405/1‑101 (MQ=255)                                                                                             ggCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGAcc                                          >  2:59677/1‑139 (MQ=255)                                                                                              gCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGAcc                                          <  2:74455/138‑1 (MQ=255)                                                                                                  aTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGc                                     >  2:182801/1‑139 (MQ=255)                                                                                                          aCCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGcac                             <  2:49207/139‑1 (MQ=255)                                                                                                          aCCGCCGGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGcac                             <  2:451110/139‑1 (MQ=255)                                                                                                            cgctgGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAg                           <  1:207078/139‑1 (MQ=255)                                                                                                              ccgGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGt                                                                                                               <  1:77930/53‑1 (MQ=255)                                                                                                              ccgGTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGt                                                                                                               >  2:77930/1‑53 (MQ=255)                                                                                                                 gTGATACCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGAt                      <  1:798950/139‑1 (MQ=255)                                                                                                                      aCCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   >  2:147785/1‑89 (MQ=255)                                                                                                                      aCCAAACCGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   <  1:147785/89‑1 (MQ=255)                                                                                                                             cGGTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCt          <  2:306670/139‑1 (MQ=255)                                                                                                                               gTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGcc                                    <  1:624238/111‑1 (MQ=255)                                                                                                                               gTACTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGcc                                    >  2:624238/1‑111 (MQ=255)                                                                                                                                  cTGCGCACACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGa     <  2:348884/139‑1 (MQ=255)                                                                                                                                     cgcacACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   >  1:845864/1‑74 (MQ=255)                                                                                                                                     cgcacACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   <  2:845864/74‑1 (MQ=255)                                                                                                                                     cgcacACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   >  2:137140/1‑74 (MQ=255)                                                                                                                                     cgcacACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCg                                                                   <  1:137140/74‑1 (MQ=255)                                                                                                                                     cgcacACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTg  >  1:522921/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGACGGTAAAGTTGATACCCGTGCGCTGGAAGAGGTCGGTCTGACCGAAGCCCAGGCACAGGAGATGTACCGTTATCTGGCGATTG  >  NZ_CP009273/1280137‑1280407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑