Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,350,506	G→T	R288S (CGT→AGT)	sapA ←	peptide ABC transporter substrate‑binding protein SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,350,506	0	G	T	100.0%	95.0 / NA	27	R288S (CGT→AGT)	sapA	peptide ABC transporter substrate‑binding protein SapA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/15);  total (12/15)

AGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCAT  >  NZ_CP009273/1350388‑1350638                                                                                                                       |                                                                                                                                     aGGCTCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCa                                                                                                                  <  1:771901/139‑1 (MQ=255)  ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTTAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAAATCAAGCGCGGGTCGTCACGCa                                                                                                                  >  1:649570/1‑138 (MQ=255)              taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATGCCAGATAGGGGACGGTCATCCCCGGGCGCAGCGGTAAACTCAAGCGCGGGGCGTCACGCAAAATGGGAAgctg                                                                                                     >  1:645794/1‑139 (MQ=255)                      aGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGgcag                                                                                             <  2:649570/139‑1 (MQ=255)                        tGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATGCCCGAAAGGGGGCGTTCATCCCAGGACGCAGCGGTAAAATCAAGCGCGGGGCGGCACGCAAAAAGGATAGCTGGGTGggag                                                                                             >  2:620428/1‑137 (MQ=255)                                cATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGcaggccaggc                                                                                   <  2:821267/139‑1 (MQ=255)                                   ggCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGTTAAATGCCCGATAAGCGGCGTTCATCCCAGGGCGCCGCGTTTAACTCAAACGCGGGGCGTCACGCAAAATGGGTAGCTGGGTGGCAGCagggccggcccg                                                                                >  1:785558/1‑137 (MQ=255)                                    gCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGgccaggccag                                                                                <  2:808604/138‑1 (MQ=255)                                                cagattTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTcc                                                                   <  1:571077/136‑1 (MQ=255)                                                attattTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTcc                                                                   <  2:698245/139‑1 (MQ=255)                                                    tttAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCg                                                                <  2:645794/138‑1 (MQ=255)                                                        gcggcgGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCa                                                            <  1:212566/138‑1 (MQ=255)                                                                         ttAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc                                          >  1:182795/1‑139 (MQ=255)                                                                         ttAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc                                          >  2:662720/1‑139 (MQ=255)                                                                         ttAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc                                          >  1:269853/1‑139 (MQ=255)                                                                              tGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTg                                     <  2:785558/139‑1 (MQ=255)                                                                                 cAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccg                                  >  2:222038/1‑139 (MQ=255)                                                                                          gACGTTCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCt                         <  1:386428/139‑1 (MQ=255)                                                                                              ttCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAgc                                                                                                       >  2:970/1‑57 (MQ=255)                                                                                              ttCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAgc                                                                                                       <  1:970/57‑1 (MQ=255)                                                                                              ttCATCCCAGGACGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAAt                     <  2:18958/139‑1 (MQ=255)                                                                                                          cGCAGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCt         <  2:182795/139‑1 (MQ=255)                                                                                                             aGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAg                                                           >  2:704301/1‑86 (MQ=255)                                                                                                             aGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAg                                                           <  1:704301/86‑1 (MQ=255)                                                                                                             aGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCg      >  1:810594/1‑139 (MQ=255)                                                                                                             aGCGTTAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCg      >  1:677267/1‑139 (MQ=255)                                                                                                                 ttAAACTCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCAt  <  1:620428/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                     AGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGCCGGAGCCTAAATCCACCACTACCTGCGGCAT  >  NZ_CP009273/1350388‑1350638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑