Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,386,326 C→T R27C (CGC→TGC)  pgrR → DNA‑binding transcriptional regulator PgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,386,3260CT100.0% 128.5 / NA 34R27C (CGC→TGC) pgrRDNA‑binding transcriptional regulator PgrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

AAATCATGATTAGACCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTG  >  NZ_CP009273/1386193‑1386461
                                                                                                                                     |                                                                                                                                       
aaaTCATGATTAGACCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAgcctgcct                                                                                                                                     >  1:682613/1‑138 (MQ=255)
     aTGATTAGACCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCAt                                                                                                                               >  2:170818/1‑139 (MQ=255)
            gACCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAg                                                                                                                        >  1:636663/1‑139 (MQ=255)
             aCCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGt                                                                                                                       <  1:579287/139‑1 (MQ=255)
                 aTCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGc                                                                                                                   >  2:845450/1‑139 (MQ=255)
                 aTCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGc                                                                                                                   >  2:613760/1‑139 (MQ=255)
                              ttAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGa                                                                                                        >  1:397607/1‑137 (MQ=255)
                              ttAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATa                                                                                                      <  1:655775/139‑1 (MQ=255)
                                    gATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  1:105086/138‑1 (MQ=255)
                                    gATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:370831/138‑1 (MQ=255)
                                    gATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  1:709603/138‑1 (MQ=255)
                                    gATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:420490/138‑1 (MQ=255)
                                    gATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:563063/138‑1 (MQ=255)
                                       tGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcgtcg                                                                                              >  2:196268/1‑138 (MQ=255)
                                       tGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:448781/135‑1 (MQ=255)
                                       tGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 >  1:448781/1‑135 (MQ=255)
                                                      aatgaaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGAtt                                                                               <  1:845450/138‑1 (MQ=255)
                                                      aatgaaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGAt                                                                                <  1:196268/137‑1 (MQ=255)
                                                      aatgaaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTg                                                                              >  1:94400/1‑139 (MQ=255)
                                                        tgaaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 >  1:119235/1‑118 (MQ=255)
                                                        tgaaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:119235/118‑1 (MQ=255)
                                                          aaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCt                                                                                                                  >  1:775470/1‑99 (MQ=255)
                                                          aaGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCt                                                                                                                  <  2:775470/99‑1 (MQ=255)
                                                                                   tGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg                                                 <  2:770305/139‑1 (MQ=255)
                                                                                       gTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGc                                             <  1:170818/139‑1 (MQ=255)
                                                                                                             cGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 >  1:730284/1‑65 (MQ=255)
                                                                                                             cGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 >  1:678015/1‑65 (MQ=255)
                                                                                                             cGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:678015/65‑1 (MQ=255)
                                                                                                             cGTAGCTTCACTCGTGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGcg                                                                                                 <  2:730284/65‑1 (MQ=255)
                                                                                                                           tGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt                                        >  2:124864/1‑108 (MQ=255)
                                                                                                                           tGCAGCAGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgt                                        <  1:124864/108‑1 (MQ=255)
                                                                                                                            gcagcaGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg                                                 <  1:809878/98‑1 (MQ=255)
                                                                                                                            gcagcaGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg                                                 >  2:809878/1‑98 (MQ=255)
                                                                                                                            gcagcaGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTTGTCTg        >  1:585444/1‑139 (MQ=255)
                                                                                                                            gcagcaGCCTGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTTGTCTg        >  1:345350/1‑139 (MQ=255)
                                                                                                                                  gcctgcctGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTg  <  2:91138/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AAATCATGATTAGACCTATCATTTAAATGATTAATAGATTGCATGGAGATACTGAATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGCTTCACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTG  >  NZ_CP009273/1386193‑1386461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑