| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,541,514 | T→C | intergenic (‑88/‑144) | yddG ← / → fdnG | aromatic amino acid efflux DMT transporter YddG/formate dehydrogenase‑N subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,541,514 | 0 | T | C | 75.0% | 47.5 / 17.4 | 28 | intergenic (‑88/‑144) | yddG/fdnG | aromatic amino acid efflux DMT transporter YddG/formate dehydrogenase‑N subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (11/10); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
AGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGC > NZ_CP009273/1541380‑1541628 | aGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTa < 1:339032/139‑1 (MQ=255) ggACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTAc < 1:79036/139‑1 (MQ=255) ggACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTAc < 2:772212/139‑1 (MQ=255) gACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTAcc > 1:102363/1‑139 (MQ=255) cccTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGaggtaggt > 1:612789/1‑139 (MQ=255) aTGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAAt > 1:253112/1‑139 (MQ=255) gTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCg > 2:781658/1‑115 (MQ=255) gTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCg < 1:781658/115‑1 (MQ=255) gCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGaggtagg > 1:384019/1‑125 (MQ=255) gCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGaggtagg < 2:384019/125‑1 (MQ=255) tgtCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTa < 1:620259/104‑1 (MQ=255) tgtCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTa > 2:620259/1‑104 (MQ=255) tgtCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGtatattac > 2:200174/1‑137 (MQ=255) tgtCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTAtttt > 2:76912/1‑139 (MQ=255) tgtCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTAtttt > 1:624972/1‑139 (MQ=255) gCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGAttt > 1:760172/1‑139 (MQ=255) aaaaaTTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCgcgc > 2:814754/1‑138 (MQ=255) aaaaaTTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCgcgc > 1:84293/1‑138 (MQ=255) aaaTTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCaaa < 2:20072/139‑1 (MQ=255) taGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACt < 1:40602/139‑1 (MQ=255) aCTGGCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTcg < 2:612789/139‑1 (MQ=255) gCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTcgcg < 1:650805/137‑1 (MQ=255) gCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCgcgc < 2:236385/138‑1 (MQ=255) gCCTGTCAGGCAGTGGTGCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCgcgc < 2:570762/138‑1 (MQ=255) gtgCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCa > 2:345462/1‑60 (MQ=255) gtgCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCa > 2:496225/1‑60 (MQ=255) gtgCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCa < 1:496225/60‑1 (MQ=255) gtgCGTTTCTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCa < 1:345462/60‑1 (MQ=255) tttctCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGAtt < 2:611717/90‑1 (MQ=255) tttctCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGAtt > 1:611717/1‑90 (MQ=255) | AGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATTAAGCTGAATTTTATAGCATTTTTTTAACTGGCCTGTCAGGCAGTGGTGCGTTTTTCTACCGCTATTGAGGTAGGTCAATTTGCGAAGGCGGATTATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGC > NZ_CP009273/1541380‑1541628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |